[Histonet] IHC pos. & neg. control question

Tony Henwood AnthonyH <@t> chw.edu.au
Thu May 19 21:47:23 CDT 2011

"either would fulfill the purpose of detecting non-specific positive staining" - NO

Not in the patients sample unless it is included (which for diagnostic uses is the most important).

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jean-Martin Lapointe
Sent: Friday, 20 May 2011 7:50 AM
To: Angela Bitting; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question

I can certainly agree with that. Whether it’s the patient’s sample or the known positive control that are stained without primary, either would fulfill the purpose of detecting non-specific positive staining. 




From: Angela Bitting [mailto:akbitting <@t> geisinger.edu] 
Sent: Thursday, May 19, 2011 4:50 PM
To: Jean-Martin Lapointe; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC pos. & neg. control question


I agree with the majority. A  patient slide stained without primary antibody, and a patient section with primary antibody and  a known positive control tissue, on the same slide when possible, is sufficient. I do agree, however, that the use of a negative tissue control is important in your initial antibody optimization and validation.


Angela Bitting, HT(ASCP), QIHC
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 


email. Thank you.

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