[Histonet] IHC pos. & neg. control question
Jean-Martin Lapointe
jm.lapointe <@t> accellab.com
Thu May 19 15:14:36 CDT 2011
I think that this is indeed what is happening here, that there is confusion between a negative control stain (positive tissue, stained without the primary ab) and a negative control tissue (tissue known to not express the marker, stained normally).
I had assumed we were talking about the former, because this is what the pathologist cited by Curt wrote in his email. While a known negative control tissue is useful in an IHC run, I am somewhat alarmed to gather from the responses sent that a section without primary is NOT being included in standard IHC runs at your respective labs. To me, this is an absolute necessity in order to properly evaluate IHC results. But that might be because I work in research rather than in a clinical setting, where i'm assuming that priorities are different.
Also, I do not agree with Glen's post, for the reasons outlined by Pete.
Jean-Martin
------------------------------
Message: 11
Date: Thu, 19 May 2011 13:37:38 -0500
From: "Sebree Linda A" <LSebree <@t> uwhealth.org>
Subject: RE: [Histonet] IHC pos. & neg. control question
To: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F0B9FD4B28C80E43996FFD4408B198AE074F6A <@t> UWHC-MAIL3.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="us-ascii"
I basically agree with you Glen. I think some people are mixing up
Negative Reagent Controls (substituting negative serum, Ab diluent, etc.
for antibody) and Negative Tissue Controls (substituting a tissue known
to be negative for the antibody being run). It CAN be confusing.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dawson,
Glen
Sent: Thursday, May 19, 2011 1:32 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
IMHO: Running any piece of tissue as a control that does not belong to
the patient being tested makes zero sense. Because it would not be from
the patient tissue being tested, how do you know if it was handled the
same as the patient tissue? For example:
1) Were they processed the same way?
2) Did the patient tissue dry out in the OR before it was delievered?
3) Was the patient tissue ever irradiated?
4) Does the patient tissue contain any of a number of substances that
could cause non-specific staining.
5) Was the patient abducted by aliens?
My point is that running a piece of tissue as a negative control that is
not even from the patient being tested throws all of the conditions that
the patient tissue was exposed to prior to and during processing out the
window. This makes NO sense.
Glen Dawson BS, HT(ASCP) & QIHC
IHC Manager
Milwaukee, WI
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Curt
Tague
Sent: Thursday, May 19, 2011 11:04 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC pos. & neg. control question
I got this email from a pathologist today. we have always run a positive
with the patient tissue and a negative, the same patient tissue, and had
no
problems. Am I missing something. Is there any documented regulation
dictating what needs to be used for the controls. In some cases if we
get
one slide of patient tissue, then we will use the pos. and neg. cont.
from
the same block but typically it's the pt. tissue that is used for the
neg.
control. Thanks for your guidance.
Email:
"I received slides on sentinel lymph node biopsies with a positive
control
on the same slide as the breast tissue, but the negative control was
just
the patient's lymph node and did not have the corresponding section used
for
the positive control. The patient's own tissue cannot be used as a
negative
control. The tissue that stained positively must serve as the negative
control without the antibody. This is critical and you need to correct
that
immediately."
Curt
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------------------------------
Message: 12
Date: Thu, 19 May 2011 14:53:57 -0400
From: Mary Helie <mary.helie <@t> yale.edu>
Subject: [Histonet] neg control
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4DD56745.3030301 <@t> yale.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
What??? News to me as well.
------------------------------
Message: 13
Date: Thu, 19 May 2011 14:01:45 -0500
From: <sgoebel <@t> mirnarx.com>
Subject: [Histonet] Slides for IHC
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D957F2A7D21959488C492A2680F9920A22448D <@t> svrexch.asuragen.us>
Content-Type: text/plain; charset="us-ascii"
So I just cut a bunch of slides for Ki-7...I am not going to be able to
stain them until Monday. I know the best thing is to put them in 4
degrees, but will it hurt them to stay at room temp for 3 days? Putting
them in the fridge gets a lot of moisture under them and they tend to
lift more. Any other suggestions...the tissue is getting low in the
block, and I don't want to recut them Monday.
Thanks
Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
------------------------------
Message: 14
Date: Thu, 19 May 2011 14:07:53 -0500
From: "Sebree Linda A" <LSebree <@t> uwhealth.org>
Subject: RE: [Histonet] Slides for IHC
To: <sgoebel <@t> mirnarx.com>, <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F0B9FD4B28C80E43996FFD4408B198AE074F6B <@t> UWHC-MAIL3.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="us-ascii"
I would air dry them well today then store them in a sealed container,
i.e. ziploc bag, slide box, in the freezer (preferable) or fridge til
Monday. They should be fine especially if you store them sealed up
tight. We keep our tonsil controls for Ki67 in slide boxes in the
freezer all the time with no problem.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
sgoebel <@t> mirnarx.com
Sent: Thursday, May 19, 2011 2:02 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Slides for IHC
So I just cut a bunch of slides for Ki-7...I am not going to be able to
stain them until Monday. I know the best thing is to put them in 4
degrees, but will it hurt them to stay at room temp for 3 days? Putting
them in the fridge gets a lot of moisture under them and they tend to
lift more. Any other suggestions...the tissue is getting low in the
block, and I don't want to recut them Monday.
Thanks
Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Thu, 19 May 2011 15:10:53 -0400
From: "Alyssa" <alyssa <@t> alliedsearchpartners.com>
Subject: [Histonet] Histology Supervisor Needed in Fort Myers
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<!&!AAAAAAAAAAAYAAAAAAAAABz0EREyw0dIgji8AanhidHCgAAAEAAAAJdYUxk9SkhEmDyvhNDo3AwBAAAAAA==@alliedsearchpartners.com>
Content-Type: text/plain; charset="us-ascii"
Position Title: Histology Supervisor
Reports To: Laboratory Director
Shift: Monday-Friday, 8am-5pm
Location:
Dermatology Pathology Laboratory for well established busy office in Fort
Myers, FL founded in 2000.
Requirements:
* ASCP certification required
* Experience leading a team of 3+ laboratory staff memebers
* Experience and knowledge of OSHA, CLIA, AHCA regulations
* The ability to implement and maintain QA/QC policies and procedures
* Oversee safety and training
* Understanding of the different lab licensing and ranges of testing
Summary:
* Responsible for 2 other employees within the laboratory
* Work with the Director of Compliance on compliancy of the lab
* Handles all laboratory compliance
* Occasionally help with day to day routine histology
Benefits:
Health Insurance, Dental Insurance, Vision Insurance. Group Life, Short Term
Disability and Long Term Disability paid by employer. Voluntary Life for
self, spouse, and child. Discount on employer products and services. PTO for
vacation, personal time, sick etc. Paid Holidays (7): New Years Day,
Memorial Day, Fourth of July, Labor Day, Thanksgiving Day, Day after
Thanksgiving Day, and Christmas Day. Bereavement Leave. Bi-annual bonuses,
performance reviews, uniform and name badge. Direct Depost & 401K with
employer contribution.
To apply:
Please send resume and salary expectations to
Alyssa <@t> alliedsearchpartners.com . At that time we will contact you to
conduct a phone screen. Thank you!
Alyssa Peterson, Candidate Recruitment
LinkedIn: http://www.linkedin.com/in/alyssapetersonasp
Allied Search Partners
T: 888.388.7571
F: 888.388.7572
www.alliedsearchpartners.com <http://www.alliedsearchpartners.com/>
<http://www.alliedsearchpartners.com/>
This email including its attachments is intended only for the confidential
use of the individual to whom it is addressed. If you are not the intended
recipient, any use, dissemination, distribution or copying of this message
or its attachments is prohibited. If you have received this message in
error, please notify us immediately, and delete this message and its
attachments permanently from your system.
------------------------------
Message: 16
Date: Thu, 19 May 2011 12:14:39 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Slides for IHC
To: "sgoebel <@t> mirnarx.com" <sgoebel <@t> mirnarx.com>,
"Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <542886.24031.qm <@t> web65704.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
They will be safe at RT
René J.
From: "sgoebel <@t> mirnarx.com" <sgoebel <@t> mirnarx.com>
To: Histonet <@t> lists.utsouthwestern.edu
Sent: Thursday, May 19, 2011 3:01 PM
Subject: [Histonet] Slides for IHC
So I just cut a bunch of slides for Ki-7...I am not going to be able to
stain them until Monday. I know the best thing is to put them in 4
degrees, but will it hurt them to stay at room temp for 3 days? Putting
them in the fridge gets a lot of moisture under them and they tend to
lift more. Any other suggestions...the tissue is getting low in the
block, and I don't want to recut them Monday.
Thanks
Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 17
Date: Thu, 19 May 2011 14:17:02 -0500
From: "Jan Shivers" <shive003 <@t> umn.edu>
Subject: Re: [Histonet] IHC pos. & neg. control question
To: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <755A9552B75D4A078C8CD61AFA379AE6 <@t> auxs.umn.edu>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
I agree 100% with Glen.
Jan Shivers
UMN VDL
----- Original Message -----
From: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, May 19, 2011 1:32 PM
Subject: RE: [Histonet] IHC pos. & neg. control question
IMHO: Running any piece of tissue as a control that does not belong to the
patient being tested makes zero sense. Because it would not be from the
patient tissue being tested, how do you know if it was handled the same as
the patient tissue? For example:
1) Were they processed the same way?
2) Did the patient tissue dry out in the OR before it was delievered?
3) Was the patient tissue ever irradiated?
4) Does the patient tissue contain any of a number of substances that could
cause non-specific staining.
5) Was the patient abducted by aliens?
My point is that running a piece of tissue as a negative control that is not
even from the patient being tested throws all of the conditions that the
patient tissue was exposed to prior to and during processing out the window.
This makes NO sense.
Glen Dawson BS, HT(ASCP) & QIHC
IHC Manager
Milwaukee, WI
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Curt
Tague
Sent: Thursday, May 19, 2011 11:04 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC pos. & neg. control question
I got this email from a pathologist today. we have always run a positive
with the patient tissue and a negative, the same patient tissue, and had
no
problems. Am I missing something. Is there any documented regulation
dictating what needs to be used for the controls. In some cases if we
get
one slide of patient tissue, then we will use the pos. and neg. cont.
from
the same block but typically it's the pt. tissue that is used for the
neg.
control. Thanks for your guidance.
Email:
"I received slides on sentinel lymph node biopsies with a positive
control
on the same slide as the breast tissue, but the negative control was
just
the patient's lymph node and did not have the corresponding section used
for
the positive control. The patient's own tissue cannot be used as a
negative
control. The tissue that stained positively must serve as the negative
control without the antibody. This is critical and you need to correct
that
immediately."
Curt
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 18
Date: Thu, 19 May 2011 12:26:32 -0700 (PDT)
From: Keith Mikoff <mikoff <@t> pacbell.net>
Subject: [Histonet] Re: Histonet Digest, Vol 90, Issue 22 2. FW: How
to remove DAB to restain with DAB (Margaryan, Naira)
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <6554.55298.qm <@t> web80606.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi Naira,
The short answer is no, it can't be done.
Unless there is a way to release the antibody from the binding site without
damaging the binding site, it can't be done. That said, somehow it should be
possible to find a reproduce-able fix for this kind of issue, by either mending
the binding site, or enhancing the activation of the already applied antibody
complex and/or chromogen. Some really cool tricks if practical or possible.
It seems like it should, maybe with the application of an mild acidic or basic
wash... with just the right reagent, the right amount, right application and at
the right pH. Something to think about.
Keith M. Mikoff, HTL (ASCP)
------------------------------
Message: 2
Date: Thu, 19 May 2011 11:55:14 -0500
From: "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>
Subject: [Histonet] FW: How to remove DAB to restain with DAB
To: "histonet-request <@t> lists.utsouthwestern.edu"
<histonet-request <@t> lists.utsouthwestern.edu>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C1BA93040C6B9A4A8D84878F93FEC36A09B60C525E <@t> CMHEXCC01MBX.childrensmemorial.org>
Content-Type: text/plain; charset="us-ascii"
Hello,
I would like to repeat my DAB staining on the same slide with DAB I run before.
I know that "acid alcohol" will remove hematoxylin but how to remove DAB?
I appreciate to any suggestion.
Thanks in advance,
Naira
------------------------------
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End of Histonet Digest, Vol 90, Issue 22
****************************************
------------------------------
Message: 19
Date: Thu, 19 May 2011 19:26:47 +0000
From: Martha Ward <mward <@t> wfubmc.edu>
Subject: [Histonet] cost to produce one block
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<B2CECB1B6665A4479056478F6DE3C4AB039448 <@t> EXCHDB3.medctr.ad.wfubmc.edu>
Content-Type: text/plain; charset="us-ascii"
I am posting this question for a co-worker. She is attempting to calculate the cost of producing one formalin-fixed, paraffin embedded block. She has the amount of personnel time it takes but is having trouble with the reagents, etc. While we know there are lots of variables she is wondering if anyone would be willing to share this information with her. She is getting differing numbers and is trying to figure out which amount is most correct. Any responses will be kept confidential.
Thanks!
Martha Ward, MT (ASCP) QIHC
Assistant Manager
Molecular Diagnostics Lab
Dept. of Pathology
Wake Forest University Baptist Medical Center
Winston-Salem, NC 27157
336-716-2104
------------------------------
Message: 20
Date: Thu, 19 May 2011 14:31:11 -0500
From: <Pete.Pedersen <@t> HealthONEcares.com>
Subject: RE: [Histonet] IHC pos. & neg. control question
To: <GDawson <@t> dynacaremilwaukee.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F8839B25F766E74282F5A527B297BBED69410969CA <@t> FWDCWPMSGCMS06.hca.corpad.net>
Content-Type: text/plain; charset="us-ascii"
Glen,
If I am to understand you correctly you are saying control tissue is not treated the same as patient tissue, therefore is useless as a negative control correct? Then inversely doesn't that mean the same thing towards the use of a positive control? How can you guarantee the positive control tissue was treated the same as the positive stained patient tissue? According to your logic it cannot. Therefore, without the use of a negative control how can you say the staining seen in the positive control is truly positive and not artifact? Best practice says use positive and negative patient and control tissue. Please enlighten me if you know anything to the contrary?
Pete Pedersen B.S. HTL (ASCP)
Anatomic Pathology Supervisor
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dawson, Glen
Sent: Thursday, May 19, 2011 12:32 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
IMHO: Running any piece of tissue as a control that does not belong to the patient being tested makes zero sense. Because it would not be from the patient tissue being tested, how do you know if it was handled the same as the patient tissue? For example:
1) Were they processed the same way?
2) Did the patient tissue dry out in the OR before it was delievered?
3) Was the patient tissue ever irradiated?
4) Does the patient tissue contain any of a number of substances that could cause non-specific staining.
5) Was the patient abducted by aliens?
My point is that running a piece of tissue as a negative control that is not even from the patient being tested throws all of the conditions that the patient tissue was exposed to prior to and during processing out the window. This makes NO sense.
Glen Dawson BS, HT(ASCP) & QIHC
IHC Manager
Milwaukee, WI
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Curt
Tague
Sent: Thursday, May 19, 2011 11:04 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC pos. & neg. control question
I got this email from a pathologist today. we have always run a positive
with the patient tissue and a negative, the same patient tissue, and had
no
problems. Am I missing something. Is there any documented regulation
dictating what needs to be used for the controls. In some cases if we
get
one slide of patient tissue, then we will use the pos. and neg. cont.
from
the same block but typically it's the pt. tissue that is used for the
neg.
control. Thanks for your guidance.
Email:
"I received slides on sentinel lymph node biopsies with a positive
control
on the same slide as the breast tissue, but the negative control was
just
the patient's lymph node and did not have the corresponding section used
for
the positive control. The patient's own tissue cannot be used as a
negative
control. The tissue that stained positively must serve as the negative
control without the antibody. This is critical and you need to correct
that
immediately."
Curt
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 21
Date: Thu, 19 May 2011 12:36:52 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] cost to produce one block
To: Martha Ward <mward <@t> wfubmc.edu>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <645466.62523.qm <@t> web65709.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Under separate cover I am sending an article on the subject of direct costs.
René J.
From: Martha Ward <mward <@t> wfubmc.edu>
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, May 19, 2011 3:26 PM
Subject: [Histonet] cost to produce one block
I am posting this question for a co-worker. She is attempting to calculate the cost of producing one formalin-fixed, paraffin embedded block. She has the amount of personnel time it takes but is having trouble with the reagents, etc. While we know there are lots of variables she is wondering if anyone would be willing to share this information with her. She is getting differing numbers and is trying to figure out which amount is most correct. Any responses will be kept confidential.
Thanks!
Martha Ward, MT (ASCP) QIHC
Assistant Manager
Molecular Diagnostics Lab
Dept. of Pathology
Wake Forest University Baptist Medical Center
Winston-Salem, NC 27157
336-716-2104
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 22
Date: Thu, 19 May 2011 12:39:25 -0700
From: "Thomas Jasper" <tjasper <@t> copc.net>
Subject: RE: [Histonet] IHC pos. & neg. control question
To: <Pete.Pedersen <@t> HealthONEcares.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<90354A475B420441B2A0396E5008D49692C02C <@t> copc-sbs.COPC.local>
Content-Type: text/plain; charset="us-ascii"
Pete,
When you run a positive control. The tissue is already a known positive
(or it should be) for whichever antibody you are running regardless of
prior handling. It would be impossible for this not to be so. However,
with a negative, the concern is seeing how the patient tissue turns out
when subjected to all the same conditions, minus the antibody.
tj
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Pete.Pedersen <@t> HealthONEcares.com
Sent: Thursday, May 19, 2011 12:31 PM
To: GDawson <@t> dynacaremilwaukee.com; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
Glen,
If I am to understand you correctly you are saying control tissue is not
treated the same as patient tissue, therefore is useless as a negative
control correct? Then inversely doesn't that mean the same thing towards
the use of a positive control? How can you guarantee the positive
control tissue was treated the same as the positive stained patient
tissue? According to your logic it cannot. Therefore, without the use of
a negative control how can you say the staining seen in the positive
control is truly positive and not artifact? Best practice says use
positive and negative patient and control tissue. Please enlighten me if
you know anything to the contrary?
Pete Pedersen B.S. HTL (ASCP)
Anatomic Pathology Supervisor
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dawson,
Glen
Sent: Thursday, May 19, 2011 12:32 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
IMHO: Running any piece of tissue as a control that does not belong to
the patient being tested makes zero sense. Because it would not be from
the patient tissue being tested, how do you know if it was handled the
same as the patient tissue? For example:
1) Were they processed the same way?
2) Did the patient tissue dry out in the OR before it was delievered?
3) Was the patient tissue ever irradiated?
4) Does the patient tissue contain any of a number of substances that
could cause non-specific staining.
5) Was the patient abducted by aliens?
My point is that running a piece of tissue as a negative control that is
not even from the patient being tested throws all of the conditions that
the patient tissue was exposed to prior to and during processing out the
window. This makes NO sense.
Glen Dawson BS, HT(ASCP) & QIHC
IHC Manager
Milwaukee, WI
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Curt
Tague
Sent: Thursday, May 19, 2011 11:04 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC pos. & neg. control question
I got this email from a pathologist today. we have always run a positive
with the patient tissue and a negative, the same patient tissue, and had
no
problems. Am I missing something. Is there any documented regulation
dictating what needs to be used for the controls. In some cases if we
get
one slide of patient tissue, then we will use the pos. and neg. cont.
from
the same block but typically it's the pt. tissue that is used for the
neg.
control. Thanks for your guidance.
Email:
"I received slides on sentinel lymph node biopsies with a positive
control
on the same slide as the breast tissue, but the negative control was
just
the patient's lymph node and did not have the corresponding section used
for
the positive control. The patient's own tissue cannot be used as a
negative
control. The tissue that stained positively must serve as the negative
control without the antibody. This is critical and you need to correct
that
immediately."
Curt
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End of Histonet Digest, Vol 90, Issue 23
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