[Histonet] Formalin fixed frozen sections

[Rene J Buesa]

Formalin fixed FS before embedding and sectioning? That is quite confuse.
Your FS should be fixed before H&E staining (acetone or even 10% NBF will do). If you refer to the specimen that was frozen to do the FS, before being embedded and cut, has to be fixed to become a FFPE specimen.
René J.

From: "Sherwood, Margaret " <MSHERWOOD <@t> PARTNERS.ORG>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wednesday, May 18, 2011 3:47 PM
Subject: Re: [Histonet] Formalin fixed frozen sections

Does anyone fix their frozen tissue first in formalin before embedding and
sectioning?  I know one investigator in our lab did that previously, but was not
sure of the strength of formalin (I don't believe it was 10%).  Another
investigator inquired about doing that.

I would appreciate hearing from fellow histonetters.

Thanks!
Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org 




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