[Histonet] Formalin fixed frozen sections

[Rathborne, Toni]

Not before embedding in OCT, but we have 10%nbf as our first station in the staining process. The slides stay in there for 1 minute, water rinse, then proceed with your stain as usual.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret 
Sent: Wednesday, May 18, 2011 3:47 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Formalin fixed frozen sections

Does anyone fix their frozen tissue first in formalin before embedding and sectioning?  I know one investigator in our lab did that previously, but was not sure of the strength of formalin (I don't believe it was 10%).  Another investigator inquired about doing that.

I would appreciate hearing from fellow histonetters.

Thanks!
Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org 




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