[Histonet] Paraffin blocks from cell line

[Bernice Frederick]

You can use Histogel as well from Richard Allan (Fisher or VWR)

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of IRENA
SREBOTNIK KIRBIS
Sent: Wednesday, May 18, 2011 4:51 AM
To: kstoll <@t> mcw.edu; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Paraffin blocks from cell line


we made a cell block from cell line by following procedure:
- centrifuge cell suspension
- fix the sediment overnight in formalin (for tissue fixation) - optional,
the button will be anyway fixed by subsequent procedure
- centrifuge and decant the formalin
- add few drops of liquid agar (not too hot)
- as the button solidify you can put it in cassette and process as
diagnostic tissue.
We got good immunoreactions on sections prepared from melanoma cell line for
example.
hope this helps
Irena Kirbis
> From: kstoll <@t> mcw.edu
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Tue, 17 May 2011 13:45:41 -0500
> Subject: [Histonet] Paraffin blocks from cell line
> 
> Hi histonetters,
> 
> Has anyone made up paraffin blocks from cells from a cell line. This is
new to me. I assume they will be in some kind of a media. We will be getting
the cells and incubating them to grow.
> Thanks in advance for the help
> 
> Kathryn Stoll, HT(ASCP)
> Depatment of Pathology
> Medical College of Wisconsin
> 9200 W Wisconsin Ave
> Milwaukee WI 53226
> 414.805.1525
> kstoll <@t> mcw.edu
> 
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