[Histonet] 100 micron paraffin sections?

[Tim Wheelock]

Hi Everyone:

A researcher in our building has done a Golgi-Kopse silver stain on some 
brain tissue.
He says that the tissue has come out brittle with this particular Golgi 
method.

His pieces are 3 mm thick and will easily fit inside of a processing 
cassette.
He would like to embed the tissue in paraffin and cut 100 micron sections.

Does anyone have a protocol for processing this kind of  tissue into 
paraffin and then sectioning and mounting such thick 100 micron sections?

Thanks,

Tim

Tim Wheelock
Assistant Director, Neuropathology
Instructor In Neuroanatomy
Harvard Brain Tissue Resource Center
203 Mailman Research Center
McLean Hospital
Belmont MA 02478
Phone: 617-855-3592
Fax: 617-855-3199