[Histonet] Paraffin blocks from cell line

[IRENA SREBOTNIK KIRBIS]

we made a cell block from cell line by following procedure:
- centrifuge cell suspension
- fix the sediment overnight in formalin (for tissue fixation) - optional, the button will be anyway fixed by subsequent procedure
- centrifuge and decant the formalin
- add few drops of liquid agar (not too hot)
- as the button solidify you can put it in cassette and process as diagnostic tissue.
We got good immunoreactions on sections prepared from melanoma cell line for example.
hope this helps
Irena Kirbis
> From: kstoll <@t> mcw.edu
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Tue, 17 May 2011 13:45:41 -0500
> Subject: [Histonet] Paraffin blocks from cell line
> 
> Hi histonetters,
> 
> Has anyone made up paraffin blocks from cells from a cell line. This is new to me. I assume they will be in some kind of a media. We will be getting the cells and incubating them to grow.
> Thanks in advance for the help
> 
> Kathryn Stoll, HT(ASCP)
> Depatment of Pathology
> Medical College of Wisconsin
> 9200 W Wisconsin Ave
> Milwaukee WI 53226
> 414.805.1525
> kstoll <@t> mcw.edu
> 
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