[Histonet] Sakura Tissue-tek Glas Air bubble problems

[Bertani, Erin]

Try an extended prime when you start it in the am, it seemed to resolve the issue with our glass coverslipper at least for now.  (We were having a horrible time with it, and that's what the repair man did.  No bubbles for five days and running!)


Erin G. Bertani, B.A.
Clinical Laboratory Assistant
Department of Pathology and Laboratory Medicine
Roswell Park Cancer Institute
Elm & Carlton Sts
Buffalo, NY 14263
Phone: 716-845-3145
Fax: 716-845-2370
erin.bertani <@t> roswellpark.org
 

 

-----Original Message-----
From: Paula Pierce [mailto:contact <@t> excaliburpathology.com] 
Sent: Tuesday, May 17, 2011 10:37 AM
To: Lees, Sara; Histonet
Subject: Re: [Histonet] Sakura Tissue-tek Glas Air bubble problems

Thin the mounting media with xylene or solvent you use.
I use Permount thinned 60/40 with xylene.
 
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
631 N Broadway
Moore, OK 73160
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com




________________________________
From: "Lees, Sara" <Sara.Lees <@t> covance.com>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Tue, May 17, 2011 9:17:37 AM
Subject: [Histonet] Sakura Tissue-tek Glas Air bubble problems

Hi All,

I am having horrible problems with air bubbles , can anyone help I have
changed the speed increased the mountant volume, clean it, kissed it,  u
name it and nothing has worked...Can anyone help


Thanks Sara




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