[Histonet] uric acid crystals in tissue for gout

[Laurie Colbert]

We receive these specimens fresh and hand-process through 100% alcohol,
xylene, and paraffin.  The crystals are water soluble, so you need to
avoid any aqueous solutions. We cut one slide for routine H&E staining
and one slide that we just deparaffinize in xylene and then coverslip
directly out of that xylene.  The pathologist looks at the unstained
slide with a polarizing lens.  If the tissue gets processed routinely
(through formalin and the graded alcohols), we still cut the two slides
and sometimes the crystals are still there.
Laurie Colbert 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Diana
McCaig
Sent: Monday, May 16, 2011 5:47 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] uric acid crystals in tissue for gout

Does anyone have a method for identifying uric acid crystals in gout on
a tissue sample.

 

Diana

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