[Histonet] uric acid crystals in tissue for gout

[Rosie Scrimizzi]

You can also do dab smears with the fresh tissue on a slide, pop a coverslip on top and examine the wet prep under a polarising microscope. I agree routine processing doesn't completely remove UA crystals and an unstained section as described below can be used to demonstrate UA

Rosie Scrimizzi
Siemens Australia

> Date: Mon, 16 May 2011 11:06:51 -0700
> From: laurie.colbert <@t> huntingtonhospital.com
> To: dmccaig <@t> ckha.on.ca; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] uric acid crystals in tissue for gout
> CC: 
> 
> We receive these specimens fresh and hand-process through 100% alcohol,
> xylene, and paraffin.  The crystals are water soluble, so you need to
> avoid any aqueous solutions. We cut one slide for routine H&E staining
> and one slide that we just deparaffinize in xylene and then coverslip
> directly out of that xylene.  The pathologist looks at the unstained
> slide with a polarizing lens.  If the tissue gets processed routinely
> (through formalin and the graded alcohols), we still cut the two slides
> and sometimes the crystals are still there.
> Laurie Colbert 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Diana
> McCaig
> Sent: Monday, May 16, 2011 5:47 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] uric acid crystals in tissue for gout
> 
> Does anyone have a method for identifying uric acid crystals in gout on
> a tissue sample.
> 
>  
> 
> Diana
> 
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