[Histonet] uric acid crystals in tissue for gout

Patrick Laurie foreightl <@t> gmail.com
Mon May 16 10:21:05 CDT 2011

Here we just process starting in 70% EtOH, cut the section on a bath
of 70% EtOH (watch out, it wrinkles very bad) and stain them in an
Eosin only stain (which is the H&E with all of the aqueous parts taken
out).  The pathologist looks at them with a polarizing filter.  Make
sure that they stay away from water!
A good article that talks about this is:
Evaluation of Crystals in Formalin-Fixed, Paraffin-Embedded Tissue
Sections for the Differential Diagnosis of Pseudogout, Gout, and
Tumoral Calcinosis
Shidham et. al. Mod Pathol 2001;14(8):806–810
Good luck!

On Mon, May 16, 2011 at 5:46 AM, Diana McCaig <dmccaig <@t> ckha.on.ca> wrote:
> Does anyone have a method for identifying uric acid crystals in gout on
> a tissue sample.
> Diana
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plaurie <@t> cellnetix.com

More information about the Histonet mailing list