[Histonet] CD79 alpha dilemma

[Jan Shivers]

Hi Craig,

I have found CD79a from Biocare (cat. # CM067C) to stain dog, cat, pig, cow, 
horse, sheep, and mouse so far.  Have not tried it on other species yet (in 
other words, I don't have any negative species results to report).

I do have to use HIER with high pH retrieval buffer (Dako; contains EDTA) to 
expose antigens (in a Biocare Decloaking Chamber/pressure cooker).  Citrate 
buffer doesn't do the job well enough.  Microwave oven antigen retrieval 
will also work, but it exposes fewer epitopes than the pressure cooker.

If you have any more questions, feel free to contact me directly.

Jan Shivers
Senior Scientist
IHC/Histology/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 <@t> umn.edu

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----- Original Message ----- 
From: "Farish, Craig" <CFarish <@t> csu.edu.au>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, May 12, 2011 8:35 PM
Subject: [Histonet] CD79 alpha dilemma


Hi folks - has anyone out there found a Cd79α clone and supplier which will 
cover multiple species reliably?
I can find clones which will stain cats and dogs (and humans), and clones 
which will cover horses, pigs, cows and primates (and humans, but not 
opossums for some reason??) but i'm yet to find one which will cross-react 
with both the large and small animals commonly encountered in a vet lab. I 
would rather not have to stock 2 versions of the same antibody if I can 
avoid it.
Any suggestions would be much appreciated.
As always, thanks to everyone who contributes to histonet,
Craig

Craig Farish
Senior Technical Officer
Veterinary Diagnostic Laboratory
School of Animal and Veterinary Sciences
Charles Sturt University
Boorooma Street
Wagga Wagga
NSW 2678
Australia
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