[Histonet] Americlear Staining Problems

[Rosie Scrimizzi]

agree...bleeding eosin is due to water on the slidesEnsure your dehydrating alcohol levels in the stainer containers are a bit higher than your Americlear (to counter evaporation during the day).....if not you will get water contamination of your clearing solution.
Rosie ScrimizziTechnical Specialist Siemens (Sakura)Australia

> Date: Thu, 5 May 2011 08:17:13 -0700
> From: laurie.colbert <@t> huntingtonhospital.com
> To: estellamireles <@t> gmail.com; Histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Americlear Staining Problems
> CC: 
> 
> It's usually due to water on the slides.  If your alcohols and
> Americlear are clean (water-free), check to see that your slides are
> totally submerged in these two reagents, and there is not water dripping
> down the slide.
> Laurie Colbert
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Stella
> Mireles
> Sent: Thursday, May 05, 2011 5:50 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Americlear Staining Problems
> 
> Good Morning Histonetters,
> Our H&E stained slides are bleeding out the eosin. No, I did not shoot
> the
> sheriff.
> Called the manufacturer, and was advised to: Give more time in the
> Alcohols,
> Use only 100% Alcohols after eosin. Change the last two Alcohols and
> Americlear. Done. Done. Done.
> Stain bleeding really improved, but still shows up on hit and miss
> cases.
> Has anyone had this problem and How did you fix it
> 
> Stella
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