[Histonet] Americlear Staining Problems

[Laurie Colbert]

It's usually due to water on the slides.  If your alcohols and
Americlear are clean (water-free), check to see that your slides are
totally submerged in these two reagents, and there is not water dripping
down the slide.
Laurie Colbert

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Stella
Mireles
Sent: Thursday, May 05, 2011 5:50 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Americlear Staining Problems

Good Morning Histonetters,
Our H&E stained slides are bleeding out the eosin. No, I did not shoot
the
sheriff.
Called the manufacturer, and was advised to: Give more time in the
Alcohols,
Use only 100% Alcohols after eosin. Change the last two Alcohols and
Americlear. Done. Done. Done.
Stain bleeding really improved, but still shows up on hit and miss
cases.
Has anyone had this problem and How did you fix it

Stella
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