[Histonet] Re: Bone ground sections

Damien Laudier dlaudier <@t> gmail.com
Thu May 5 08:27:44 CDT 2011

Hi Cindy,

There is really no "best" hematoxylin,as any of the popular commercial
formulations can be used successfully on ground sections. The key to
reducing/eliminating the precipitation problem is filtering the hematoxylin
really well before staining and sonicating your sections after staining. For
darker nuclear staining,I like Gill III or Ehrlich's. The Celestine
Blue-Hematoxylin technique also works well on ground sections.

-Damien L.

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