[Histonet] Stupid Rabbit primaries!

[Margaret Blount]

Having read all the other comments, here's my 2.5 pence worth! Have you
tried a background reducing antibody diluent? You can get these from
DAKO or Menarini Diagnostics:
http://www.dako.com/uk/ar38/p107410/prod_products.htm.

Or MP-905-25 / MP-905-100, depending on the volume you require, from
Menarini Diagnostics, website
http://www.dako.com/uk/ar38/p107410/prod_products.htm.

I found these useful for rabbit polyclonals.

Good luck

Margaret

Miss Margaret Blount
Histology Manager
Metabolic Research Laboratories
Level 4 Institute of Metabolic Science
Box 289, Addenbrooke's Hospital
Hills Road, Cambridge, CB2 0QQ

Tel 01223 769061/336079

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
sgoebel <@t> mirnarx.com
Sent: 22 March 2011 21:04
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Stupid Rabbit primaries!

So I haven't had to deal with rabbit polyclonal primaries in a long time
because I remember how much the background sucks with them.
Unfortunately the only available antibody is a rabbit polyclonal.  Does
anyone have any suggestions for how to eliminate the background?  I have
diluted almost to the point of the antigens not showing!  Thanks guys
and gals!!

 

Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

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