[Histonet] Stupid Rabbit primaries!

Margaret Blount mab70 <@t> medschl.cam.ac.uk
Wed Mar 23 03:31:38 CDT 2011

Having read all the other comments, here's my 2.5 pence worth! Have you
tried a background reducing antibody diluent? You can get these from
DAKO or Menarini Diagnostics:

Or MP-905-25 / MP-905-100, depending on the volume you require, from
Menarini Diagnostics, website

I found these useful for rabbit polyclonals.

Good luck


Miss Margaret Blount
Histology Manager
Metabolic Research Laboratories
Level 4 Institute of Metabolic Science
Box 289, Addenbrooke's Hospital
Hills Road, Cambridge, CB2 0QQ

Tel 01223 769061/336079

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
sgoebel <@t> mirnarx.com
Sent: 22 March 2011 21:04
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Stupid Rabbit primaries!

So I haven't had to deal with rabbit polyclonal primaries in a long time
because I remember how much the background sucks with them.
Unfortunately the only available antibody is a rabbit polyclonal.  Does
anyone have any suggestions for how to eliminate the background?  I have
diluted almost to the point of the antigens not showing!  Thanks guys
and gals!!


Sarah Goebel, BA, HT(ASCP)


Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912


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