[Histonet] RE: Stupid Rabbit primaries!

[Morken, Timothy]

Sarah, is it a whole serum antibody? Jackson Laboratories has Ig fragments that can be used to block endogenous Ig sites in the tissue and largely eliminate non-specific staining. 

A well-purified antibody prep should not give much background, and if it is affinity purified it should be almost as good as a monoclonal. 

Tim Morken
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of sgoebel <@t> mirnarx.com [sgoebel <@t> mirnarx.com]
Sent: Tuesday, March 22, 2011 2:04 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Stupid Rabbit primaries!

So I haven't had to deal with rabbit polyclonal primaries in a long time
because I remember how much the background sucks with them.
Unfortunately the only available antibody is a rabbit polyclonal.  Does
anyone have any suggestions for how to eliminate the background?  I have
diluted almost to the point of the antigens not showing!  Thanks guys
and gals!!



Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912



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