[Histonet] RE: Stupid Rabbit primaries!
Timothy.Morken <@t> ucsfmedctr.org
Tue Mar 22 21:00:25 CDT 2011
Sarah, is it a whole serum antibody? Jackson Laboratories has Ig fragments that can be used to block endogenous Ig sites in the tissue and largely eliminate non-specific staining.
A well-purified antibody prep should not give much background, and if it is affinity purified it should be almost as good as a monoclonal.
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of sgoebel <@t> mirnarx.com [sgoebel <@t> mirnarx.com]
Sent: Tuesday, March 22, 2011 2:04 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Stupid Rabbit primaries!
So I haven't had to deal with rabbit polyclonal primaries in a long time
because I remember how much the background sucks with them.
Unfortunately the only available antibody is a rabbit polyclonal. Does
anyone have any suggestions for how to eliminate the background? I have
diluted almost to the point of the antigens not showing! Thanks guys
Sarah Goebel, BA, HT(ASCP)
2150 Woodward Street
Austin, Texas 78744
(512)901-0900 ext. 6912
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