[Histonet] RE: Stupid Rabbit Primary Antibodies

Goins, Tresa TGoins <@t> mt.gov
Wed Mar 23 15:00:33 CDT 2011

You might try Background Sniper from Biocare or Background Buster from Innovex - we process animal tissues exclusively and we use several rabbit primary antibodies.

Tresa Goins, Ph.D.
Histopathology Supervisor
Department of Livestock
Bozeman, Montana

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of sgoebel <@t> mirnarx.com
Sent: Tuesday, March 22, 2011 3:04 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Stupid Rabbit primaries!

So I haven't had to deal with rabbit polyclonal primaries in a long time
because I remember how much the background sucks with them.
Unfortunately the only available antibody is a rabbit polyclonal.  Does
anyone have any suggestions for how to eliminate the background?  I have
diluted almost to the point of the antigens not showing!  Thanks guys
and gals!!


Sarah Goebel, BA, HT(ASCP)


Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912


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