[Histonet] RE: Stupid Rabbit Primary Antibodies
TGoins <@t> mt.gov
Wed Mar 23 15:00:33 CDT 2011
You might try Background Sniper from Biocare or Background Buster from Innovex - we process animal tissues exclusively and we use several rabbit primary antibodies.
Tresa Goins, Ph.D.
Department of Livestock
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of sgoebel <@t> mirnarx.com
Sent: Tuesday, March 22, 2011 3:04 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Stupid Rabbit primaries!
So I haven't had to deal with rabbit polyclonal primaries in a long time
because I remember how much the background sucks with them.
Unfortunately the only available antibody is a rabbit polyclonal. Does
anyone have any suggestions for how to eliminate the background? I have
diluted almost to the point of the antigens not showing! Thanks guys
Sarah Goebel, BA, HT(ASCP)
2150 Woodward Street
Austin, Texas 78744
(512)901-0900 ext. 6912
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