[Histonet] RE: comprehensive list?

[shive003 <@t> umn.edu]

I've also found that occasionally the antigen retrieval method quoted on a 
vendor's data sheet doesn't unmask antigens as well as another method tried 
in my initial antibody validation trials with that antibody. So, my point 
is... any comprehensive list should be used only as a guideline; results in 
your lab will be affected by the reagents, equipment, and environmental 
conditions you employ in your own lab.

Jan Shivers
UMN Vet Diag Lab


On Mar 18 2011, Truscott, Tom wrote:

 Yes, vendor sheets are good, but not all give enough info, and you may 
have to compare several vendor, and as you say, antibodies only tested by 
western blot are common in research. Yet, it would be a great money and 
timesaver if there was one site to go to, to see if anyone had tried these 
antibodies by different methods and could also submit their own results for 
entry, possibly with links to Journal articles, much like what some vendors 
do. It could be a quick reference guide to setting up some research or 
double staining methods, or just see if some new request is going to fit 
your routine. Someone would have to have more time and computer skills than 
I. Thanks all for the input, TOM T
>
>-----Original Message-----
 From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Morken, 
Timothy
>Sent: Friday, March 18, 2011 10:38 AM
>To: Histonet
>Subject: [Histonet] RE: comprehensive list?
>
>Tom, 
>
 Vendor datasheets are the best source of information. The vast majority of 
clinical vendors antibodies are designed for formalin fixed paraffin 
embedded tissue. Research vendors are all over the map and often only test 
by western blots or elisa so they may or may not have information about how 
their antibodies work in fixed tissue.
>
>Tim Morken
>Supervisor, Histology, IPOX
>UCSF Medical Center
>San Francisco, CA, USA
>
>
>-----Original Message-----
 From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Truscott, 
Tom
>Sent: Friday, March 18, 2011 9:02 AM
>To: Histonet
>Subject: [Histonet] comprehensive list?
>
 Hi All, Is there a comprehensive list in one location somewhere that gives 
information on all or most antibodies on how they work with FFPE or FS or 
other fixatives, so that we don't have to try to many approaches when we 
get a new antibody to try. Thanks, Tom T
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