[Histonet] RE: comprehensive list?

[Morken, Timothy]

Tom, No one is collecting detailed info from a lot of people's experiences, if that is what you want. Probably the closest you can get is at nordiqc site (wwww.nordiqc.org, protein atlas). They test a lot of antibodies and give some info on use and stain pattern but are by no means always reliable and certainly have not tested all antibodies. 

IHC World is another site that has some info, but not very organized in the terms you mention.


Your database idea sounds great. Are you planning on starting one?!?!!

Tim Morken
Supervisor, Histology, IPOX
UCSF Medical Center
San Francisco, CA, USA

-----Original Message-----
From: Truscott, Tom [mailto:ttruscot <@t> vetmed.wsu.edu] 
Sent: Friday, March 18, 2011 11:09 AM
To: Morken, Timothy; Histonet
Subject: RE: comprehensive list?

Yes, vendor sheets are good, but not all give enough info, and you may have to compare several vendor,  and as you say, antibodies only tested by western blot are common in research. Yet, it would be a great money and timesaver if there was one site to go to, to see if anyone had tried these antibodies by different methods and could also submit their own results for entry, possibly with links to Journal articles, much like what some vendors do. It could be a quick reference guide to setting up some research or double staining methods, or just see if some new request is going to fit your routine. Someone would have to have more time and computer skills than I. Thanks all for the input, TOM T

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Friday, March 18, 2011 10:38 AM
To: Histonet
Subject: [Histonet] RE: comprehensive list?

Tom, 

Vendor datasheets are the best source of information. The vast majority of clinical vendors antibodies are designed for formalin fixed paraffin embedded tissue. Research vendors are all over the map and often only test by western blots or elisa so they may or may not have information about how their antibodies work in fixed tissue.

Tim Morken
Supervisor, Histology, IPOX
UCSF Medical Center
San Francisco, CA, USA


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Truscott, Tom
Sent: Friday, March 18, 2011 9:02 AM
To: Histonet
Subject: [Histonet] comprehensive list?

Hi All, Is there a comprehensive list in one location somewhere that gives information on all or most antibodies on how they work with FFPE or FS or other fixatives, so that we don't have to try to many approaches when we get a new antibody to try. Thanks, Tom T
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