[Histonet] RE: comprehensive list?

[Liz Chlipala]

Vendor sheets may not be accurate we keep an internal log of all of our
antibodies and what species we have tested them in.  So far in our lab
we have found several antibodies that the vendor states works in a
species and it does not in our hands, in these cases we always contact
the vendor, basically for a refund and they have updated their
specification sheets to reflect the change.  We do use protein atlas a
lot for verification of the IHC protocols and tissue staining patterns.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
shive003 <@t> umn.edu
Sent: Friday, March 18, 2011 1:06 PM
To: Truscott, Tom
Cc: Histonet; Morken,Timothy
Subject: Re: [Histonet] RE: comprehensive list?

I've also found that occasionally the antigen retrieval method quoted on
a 
vendor's data sheet doesn't unmask antigens as well as another method
tried 
in my initial antibody validation trials with that antibody. So, my
point 
is... any comprehensive list should be used only as a guideline; results
in 
your lab will be affected by the reagents, equipment, and environmental 
conditions you employ in your own lab.

Jan Shivers
UMN Vet Diag Lab


On Mar 18 2011, Truscott, Tom wrote:

 Yes, vendor sheets are good, but not all give enough info, and you may 
have to compare several vendor, and as you say, antibodies only tested
by 
western blot are common in research. Yet, it would be a great money and 
timesaver if there was one site to go to, to see if anyone had tried
these 
antibodies by different methods and could also submit their own results
for 
entry, possibly with links to Journal articles, much like what some
vendors 
do. It could be a quick reference guide to setting up some research or 
double staining methods, or just see if some new request is going to fit

your routine. Someone would have to have more time and computer skills
than 
I. Thanks all for the input, TOM T
>
>-----Original Message-----
 From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Morken, 
Timothy
>Sent: Friday, March 18, 2011 10:38 AM
>To: Histonet
>Subject: [Histonet] RE: comprehensive list?
>
>Tom, 
>
 Vendor datasheets are the best source of information. The vast majority
of 
clinical vendors antibodies are designed for formalin fixed paraffin 
embedded tissue. Research vendors are all over the map and often only
test 
by western blots or elisa so they may or may not have information about
how 
their antibodies work in fixed tissue.
>
>Tim Morken
>Supervisor, Histology, IPOX
>UCSF Medical Center
>San Francisco, CA, USA
>
>
>-----Original Message-----
 From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Truscott, 
Tom
>Sent: Friday, March 18, 2011 9:02 AM
>To: Histonet
>Subject: [Histonet] comprehensive list?
>
 Hi All, Is there a comprehensive list in one location somewhere that
gives 
information on all or most antibodies on how they work with FFPE or FS
or 
other fixatives, so that we don't have to try to many approaches when we

get a new antibody to try. Thanks, Tom T
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