[Histonet] Bielschowskys Stain
BMolinari <@t> heart.thi.tmc.edu
Wed Mar 16 05:50:04 CDT 2011
Thank you for your help. I appreciate it.
From: Glawon Flood [mailto:smoothw <@t> gmail.com]
Sent: Tuesday, March 15, 2011 2:33 PM
To: Molinari, Betsy
Subject: Re: [Histonet] Bielschowskys Stain
I don't know of any modifications for Eager's but there are modifications for the Fink-Heimer technique as well as the Nauta-Gygax technique that can be used on paraffin-embedded tissue. I've only come across these in passing and you may have to do a little digging to find a protocol.
I hope the info proves useful...good luck!
Glawon Flood, HTL (ASCP)
On Mar 15, 2011, at 11:43 AM, "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu> wrote:
> Thank you for your response. I "googled" Eagers and found a protocol for Eagers for frozen sections. I have paraffin blocks.
> Do you know if there is and Eagers method for paraffin?
> Thank you so much.
> -----Original Message-----
> From: Glawon Flood [mailto:smoothw <@t> gmail.com]
> Sent: Tuesday, March 15, 2011 10:22 AM
> To: Molinari, Betsy
> Subject: Re: [Histonet] Bielschowskys Stain
> Hi Betsy,
> Let me start off by saying that I am by no means an expert on what's preferred. However, in my experience, I've come to find the Bielschowsky method very useful for staining nerve fibers, but I would not consider it so for demonstrating axonal degeneration. I would suggest that you maybe consider Eager's method for degenerating axons. Unlike Bielschowsky's, Eager's actually distinguishes those fibers that are normal from those that are degenerating.
> I hope this helps.
> Glawon Flood, HTL (ASCP)
> On Mar 15, 2011, at 6:00 AM, "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu> wrote:
>> Hi ,
>> An investigator would like show axonal degeneration in peripheral nerves. Is Bielschowsky's the preferred stain?
>> Betsy Molinari HT(ASCP)
>> Texas Heart Institute
>> Cardiovascular Pathology
>> 6770 Bertner Ave.
>> MC 1-283
>> Houston, TX 77030
>> 832-355-6812 (fax)
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
More information about the Histonet