[Histonet] Light hematoxylin staining in H&E

Greg Dobbin gvdobbin <@t> ihis.org
Wed Mar 16 05:44:03 CDT 2011

Are you soaking your blocks in dilute ammonium hydroxide before sectioning? Soaking too long will affect stain quality. 

Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

"I find that the harder I work, the 
more luck I seem to have."
- Thomas Jefferson

>>> "Jordan, Velma" <velma.jordan <@t> gwu-hospital.com> 03/15/11 22:22 PM >>>
We have been troubleshooting complaints of hematoxylin staining light in our H&E. The main complaint is no differentiation between the nucleus and cytoplasm. We are using Richard-Allan hematoxylin ,eosin,clarifier and bluing. We tried surgipath, changing times, pH the H2O and any other thing we could think of, but the problem remains.  Does anyone have a suggestion?  Velma Jordan

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