[Histonet] Storing slides in buffer

[Stacy Deppeler]

Hi guys,

I am wondering if anyone has much experience storing slides in buffer
(PBS-T) after retrieval for up to about 48hrs at 4C? Is it likely that they
will still stain? Or is it going to be entirely dependent on the epitope?

I'm optimizing research antibodies right now and I'd like to decrease my
turn around time by knocking off the HIER at an earlier time point.

Thanks.


-- 
Stacy Deppeler.

Research Assistant
Department of Ophthalmology
King Abdulaziz University Hospital
Riyadh, KSA