[Histonet] Storing slides in buffer

[sgoebel <@t> mirnarx.com]

I just did this last week.  I stored them in wash buffer overnight in
the frig. and they were fine =)

Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Stacy
Deppeler
Sent: Wednesday, March 16, 2011 3:31 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Storing slides in buffer

Hi guys,

I am wondering if anyone has much experience storing slides in buffer
(PBS-T) after retrieval for up to about 48hrs at 4C? Is it likely that
they
will still stain? Or is it going to be entirely dependent on the
epitope?

I'm optimizing research antibodies right now and I'd like to decrease my
turn around time by knocking off the HIER at an earlier time point.

Thanks.


-- 
Stacy Deppeler.

Research Assistant
Department of Ophthalmology
King Abdulaziz University Hospital
Riyadh, KSA
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