[Histonet] Light hematoxylin staining in H&E

[Jordan, Velma]

We have been troubleshooting complaints of hematoxylin staining light in our H&E. The main complaint is no differentiation between the nucleus and cytoplasm. We are using Richard-Allan hematoxylin ,eosin,clarifier and bluing. We tried surgipath, changing times, pH the H2O and any other thing we could think of, but the problem remains.  Does anyone have a suggestion?  Velma Jordan


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