[Histonet] Control Slides
hfedor <@t> jhmi.edu
Tue Mar 15 08:31:37 CDT 2011
Hello, We have been storing our slides in small zip loc bags at -20 and have data suggesting that slides stored in this fashion are reasonably well preserved after 3- 5 years. This was done with TMA slides. Was not done for special stain slides but for IHC.
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of pruegg <@t> ihctech.net
Sent: Tuesday, March 15, 2011 8:56 AM
To: Harrison,Sandra C.
Cc: histonet <@t> lists.utsouthwestern.edu; Amador, Amanda
Subject: RE: [Histonet] Control Slides
I do not melt dry my cut control slides until i need to use them,=d
i do store them in boxes at 4c, i have recently run into this problem
=th blocks, so now i seal all my blocks with paraffin and store
them in th�ridge as well.
-------- Original Message --------
Subject: RE:=istonet] Control Slides
From: "Harrison, Sandra C." <Sandra.Harrison3 <@t> va.gov>
Date: Mon,=rch 14, 2011 1:40 pm
To: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>, "Amador, Amanda"
&l=aamador <@t> ameripath.com>, &=;histonet <@t> lists.utso uthwestern.edu>
We dry the control slide at the same time tha=e dry the tissue
stained, since controls are supposed to be han=ed in the same
the test tissue. That being said, I guess if = were truly following
that rule, we would cut the control on the same �y we cut the test
"Other people say that, after they dry the slide, they dip the slide in paraffin, to cover the
that the air doesn't touch the tissue during the months before=e
used in a stain."
From: histone=bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.e=] On Behalf Of
Sent: Thursday, March 10, 21 6:39 PM
To: Amador, Amanda; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histone= Control Slides
I've noticed that our spirochete control slides do=t stain as
starting about 3 months after they are sectioned, =d that they stop
staining by about 6 months. (This is with a silver s=in.)
I've talked with other histotechs, and they say they've seen =e
phenomenon with AFB and Gram controls. (We use ours up too qui=ly -
never have 6-12 months old control slides for these, so I can=
Bancroft's book talks about this phenome=n with amyloid, and
oxidation of the tissue (protein= due to exposure to air may be the
I'm guessing the it prob�ly applies to the precut microorganism
We only cut enough slides for 6 months, and date them. Other people
put their cut control slides in a slide box with a lid after dryi=,
then place the box in the refrig, as cold slows down the che=cal
and the lid keeps the moving air off the slide. Other =ople say
they dry the slide, they dip the slide in par�fin, to cover the
that the air doesn't touch the tissue=ring the months before the
used in a stain. Just 3 sugge=ions to stop this problem.
Peggy A. Wenk, HTL(ASCP)SLS
Royal Oak, MI 48073
From: "Amador, Amanda" <aamador <@t> ameripath.com>
Sent: Wednesday, March 09, 2010:22 PM
To: <=stonet <@t> lists.utsouthwestern.edu>
Subject: [Histonet] Control Sl=es
> Is there guidelines for how long special stains controls a= good
> they are cut? We have spirochetes for our Stei=r that is from
> we are having issues.
>=anda Amador, HT(ASCP)CM
> AmeriPath | Histology Group Lead/Trainer=560 N Shadeland Ave,
> Indianapolis, IN 46219 | phon�17.275.8052 |
> aamador@=eripath.com<mailto:aam�or <@t> ameripath.com>; |
> _______________________________________________ > Histonet mailing list
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