[Histonet] Control Slides

[Helen Fedor]

Hello,  We have been storing our slides in small zip loc bags at -20 and have data suggesting that slides stored in this fashion are reasonably well preserved after 3- 5 years. This was done with TMA slides. Was not done for special stain slides but for IHC. 


Helen

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of pruegg <@t> ihctech.net
Sent: Tuesday, March 15, 2011 8:56 AM
To: Harrison,Sandra C.
Cc: histonet <@t> lists.utsouthwestern.edu; Amador, Amanda
Subject: RE: [Histonet] Control Slides


   I do not melt dry my cut control slides until i need to use them,=d
   i  do store them in boxes at 4c, i have recently run into this problem
   =th  blocks,  so  now i seal all my blocks with paraffin and store
   them in th�ridge as well.



   Patsy



   -------- Original Message --------
   Subject: RE:=istonet] Control Slides
   From: "Harrison, Sandra C." <[1]Sandra.Harrison3 <@t> va.gov>
   Date: Mon,=rch 14, 2011 1:40 pm
   To: "Lee & Peggy Wenk" <[2]lpwenk <@t> sbcglobal.net>, "Amador, Amanda"
   &l=[3]aamador <@t> ameripath.com>,    &=;[4]histonet <@t> lists.utso   uthwestern.edu>
   We  dry  the  control  slide at the same time tha=e dry the tissue
   being
   stained,  since  controls  are  supposed  to  be han=ed in the same
   manner as
   the test tissue. That being said, I guess if = were truly following
   that rule, we would cut the control on the same �y we cut the test
   sample. :-)
   "Other people say that, after   they  dry  the  slide,  they  dip  the slide in paraffin, to cover the
   tissu=
   so
   that  the  air  doesn't touch the tissue during the months before=e
   slide
   is
   used in a stain."
   -----Original Message-----
   From: [5]histone=bounces <@t> lists.utsouthwestern.edu
   [[6]mailto:histonet-bounces <@t> lists.utsouthwestern.e=]  On Behalf Of
   Lee &
   Peggy Wenk
   Sent: Thursday, March 10, 2
1 6:39 PM
   To: Amador, Amanda; [7]histonet <@t> lists.utsouthwestern.edu
   Subject: Re: [Histone= Control Slides
   I've  noticed  that  our  spirochete  control  slides do=t stain as
   intense
   starting about 3 months after they are sectioned, =d that they stop
   staining by about 6 months. (This is with a silver s=in.)
   I've  talked  with  other  histotechs, and they say they've seen =e
   same
   phenomenon with AFB and Gram controls. (We use ours up too qui=ly -
   we
   never  have  6-12  months  old  control slides for these, so I can=
   attest
   to
   this. )
   Bancroft's  book  talks  about  this  phenome=n  with amyloid, and
   suggests
   that
   oxidation of the tissue (protein= due to exposure to air may be the
   cause.
   I'm  guessing  the  it  prob�ly applies to the precut microorganism
   control
   slides, too.
   We  only  cut  enough slides for 6 months, and date them. Other people
   say
   they
   put their cut control slides in a slide box with a lid after dryi=,
   and
   then place the box in the refrig, as cold slows down the che=cal
   change,
   and  the  lid  keeps  the moving air off the slide. Other =ople say
   that,
   after
   they  dry  the  slide,  they dip the slide in par�fin, to cover the
   tissue,
   so
   that  the  air  doesn't touch the tissue=ring the months before the
   slide
   is
   used in a stain. Just 3 sugge=ions to stop this problem.
   Peggy A. Wenk, HTL(ASCP)SLS
   Beaumont=spital
   Royal Oak, MI 48073
   -----------------------------------=-------------
   From: "Amador, Amanda" <[8]aamador <@t> ameripath.com>
   Sent: Wednesday, March 09, 2010:22 PM
   To: <[9]=stonet <@t> lists.utsouthwestern.edu>
   Subject: [Histonet] Control Sl=es
   >  Is there guidelines for how long special stains controls a= good
   for
   once
   >  they  are  cut?  We have spirochetes for our Stei=r that is from
   2007
   and
   > we are having issues.
   >
   >=anda Amador, HT(ASCP)CM
   >  AmeriPath  |  Histology  Group Lead/Trainer=560 N Shadeland Ave,
   Suite
   A |
   > Indianapolis, IN 46219 | phon�17.275.8052 |
   > [10]aamador@=eripath.com<mailto:[11]aam�or <@t> ameripath.com>; |
   > [12]www.AmeriPath.com<http://www.ameripath.c=/>;
   >
   > _______________________________________________   > Histonet mailing list
   > [13]Histonet <@t> lists.utsouthwestern.edu
   > [14]http://lists.utso=hwestern.edu/mailman/listinfo/histonet
   __________________=___________________________
   Histonet mailing list
   [15]Histonet <@t> lists.utsouthwestern.edu[16]htt   p://lists.utsouthwestern.edu/mailman/listinfo/histonet
   _________=____________________________________
   Histonet mailing list
   [17]Histonet <@t> lists.utsouthwestern=du
   [18]http://lists.utsouthwestern.edu/mailman/listinfo/histonet
   

References

   1. file://localhost/tmp/3D"ma   2. file://localhost/tmp/3D"mailto   3. 3D"mailto:aamador <@t> ameripath.com"
   4. 3D"mailto:histonet <@t> lists.utsouthwestern.edu"
   5. 3D"mailto:histonet-bounces <@t> lists.utsouthwestern.edu"
   6. 3D"mailto:histonet-bounc   7. 3D"mailto:histonet <@t> lists.utsouth   8. 3D"mailto:aamador <@t> ame   9. 3D"mailto:histonet <@t> lists.utsouthwestern.edu"
  10. 3D"mailto:aamador <@t> ameripath.com"
  11. 3D"http://aamador@ameripath.com>"/
  12. 3D"http://www.AmeriPath.com&l=
  13. 3D"mailto:Histonet <@t> lists.uts  14. file://localhost/tmp/3D"h  15. file://localhost/tmp/3D"mailto  16. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"
  17. ="mailto:Histonet <@t> lists.utsouthwestern.edu"
  18. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/his_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet