[Histonet] immunostaining question
Rene J Buesa
rjbuesa <@t> yahoo.com
Mon Mar 14 16:01:53 CDT 2011
Yes you can, especially if the antigen is not nuclear.
--- On Mon, 3/14/11, Pop Elena <med_leni <@t> yahoo.com> wrote:
From: Pop Elena <med_leni <@t> yahoo.com>
Subject: [Histonet] immunostaining question
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, March 14, 2011, 4:57 PM
I am trying some new antibodies on parafin embeded radical prostactectomies
tissue sections and I started with the highest recomended dilution but it seems
I did not get any staining. I already counterstained the slides w/ hematoxylin
and I was wondering if it's possible to go a few steps back and incubate again
with a higher concentration of the antibody? I have a limited amount of tissue
sections for "testing".
Any imput is appreciated!
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
More information about the Histonet