[Histonet] immunostaining question

Rene J Buesa rjbuesa <@t> yahoo.com
Mon Mar 14 16:01:53 CDT 2011

Yes you can, especially if the antigen is not nuclear.
René  J.

--- On Mon, 3/14/11, Pop Elena <med_leni <@t> yahoo.com> wrote:

From: Pop Elena <med_leni <@t> yahoo.com>
Subject: [Histonet] immunostaining question
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, March 14, 2011, 4:57 PM

Hello everybody,

I am trying some new antibodies on parafin embeded radical prostactectomies 
tissue sections and I started with the highest recomended dilution but it seems 
I did not get any staining. I already counterstained the slides w/ hematoxylin 
and I was wondering if it's possible to go a few steps back and incubate again 
with a higher concentration of the antibody? I have a limited amount of tissue 
sections for "testing".

Any imput is appreciated!

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