[Histonet] immunostaining question
med_leni <@t> yahoo.com
Mon Mar 14 15:57:48 CDT 2011
I am trying some new antibodies on parafin embeded radical prostactectomies
tissue sections and I started with the highest recomended dilution but it seems
I did not get any staining. I already counterstained the slides w/ hematoxylin
and I was wondering if it's possible to go a few steps back and incubate again
with a higher concentration of the antibody? I have a limited amount of tissue
sections for "testing".
Any imput is appreciated!
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