[Histonet] immunostaining question

Pop Elena med_leni <@t> yahoo.com
Mon Mar 14 15:57:48 CDT 2011

Hello everybody,

I am trying some new antibodies on parafin embeded radical prostactectomies 
tissue sections and I started with the highest recomended dilution but it seems 
I did not get any staining. I already counterstained the slides w/ hematoxylin 
and I was wondering if it's possible to go a few steps back and incubate again 
with a higher concentration of the antibody? I have a limited amount of tissue 
sections for "testing".

Any imput is appreciated!


More information about the Histonet mailing list