[Histonet] immunostaining question

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Hello everybody,

I am trying some new antibodies on parafin embeded radical prostactectomies 
tissue sections and I started with the highest recomended dilution but it seems 
I did not get any staining. I already counterstained the slides w/ hematoxylin 
and I was wondering if it's possible to go a few steps back and incubate again 
with a higher concentration of the antibody? I have a limited amount of tissue 
sections for "testing".

Any imput is appreciated!
Thanks,
Leni