[Histonet] immunostaining question
Sebree Linda A
LSebree <@t> uwhealth.org
Tue Mar 15 07:35:51 CDT 2011
Yes, most definitely.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
600 Highland Ave.
Madison, WI 53792
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pop
Sent: Monday, March 14, 2011 3:58 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] immunostaining question
I am trying some new antibodies on parafin embeded radical
tissue sections and I started with the highest recomended dilution but
I did not get any staining. I already counterstained the slides w/
and I was wondering if it's possible to go a few steps back and incubate
with a higher concentration of the antibody? I have a limited amount of
sections for "testing".
Any imput is appreciated!
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