[Histonet] Macrophage marker in sheep for IHC

[Vivek Saroha]

Thank you to everyone who has replied and given suggestions.

I now have some ideas to help me start and will update and acknowledge once again once I make some progress.


Best regards

Vivek

-----Original Message-----
From: Jan Shivers [mailto:shive003 <@t> umn.edu] 
Sent: 24 June 2011 14:37
To: Vivek Saroha; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Macrophage marker in sheep for IHC

CD68 - clone KP1 has not worked on sheep (also not dogs, cows, horses, and 
chickens) in my experience.  I have found it to stain primates well (and 
rare cells in pigs and cats).

Jan Shivers
Senior Scientist
Histology/IHC/EM Section Head
Pathology Teaching Program
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 <@t> umn.edu

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----- Original Message ----- 
From: "Vivek Saroha" <Vivek.Saroha <@t> nottingham.ac.uk>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, June 24, 2011 4:05 AM
Subject: [Histonet] Macrophage marker in sheep for IHC


Hi there,
I am very new to basic sciences research and am planning an experiment to 
quantify and compare adipose tissue macrophage infiltration in sheep model 
using immunohistochemistry. I am specifically interested in CD11c as a 
marker of M1 activation of Macrophages. I have been advised that it is not 
easy and previous effort by my predecessors have not been very successful in 
this!
>From the general google, histonet/histosearch and pubmed search. I have 
>made a list of potential candidates:

1.    Clones PG M1 and Clone KP1
(Source : Histonet : 
http://www.histosearch.com/histonet/Nov03A/RE.HistonetSeepmacrophage.html)


2.    EMB11, a mouse anti-human CD68 mAb (Dako, Carpenteria, CA)
(source: published paper: http://www.ncbi.nlm.nih.gov/pubmed/16460804 )


 1.  Another CD68 antibody
Source: 
http://www.ingentaconnect.com/content/ocean/ajra/2011/00000025/00000002/art00024)

 1.  Iba1 (ionized calcium binding adaptor molecule 1) is a 17-kDa EF hand 
protein that is specifically expressed in macrophages/microglia and is 
upregulated during the activation of these cells.[Wikipedia]
(source: I do not remember where I found this one from but Abcam has several 
antibodies none of which are predicted for sheep)
I am writing to ask for suggestions about which one to go for and any other 
tips about searching for antibodies or performing IHC in paraffin fixed 
adipose tissue/liver.
Any ideas will be gratefully appreciated as I am very new and isolated in 
this aspect.
Best wishes
Vivek

Dr Vivek Saroha
Clinical lecturer in Child health
University of Nottingham
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