[Histonet] Tissue Shriveling in Paraffin

Michelle Aono aonomic <@t> auburn.edu
Fri Jun 24 08:27:33 CDT 2011


I was cutting some bone/joint tissue and noticed that the cartilaginous
portion was concave/indented, instead of flush with the rest of the
block surface.  Even as I continued to cut that portion always seemed a
little sunken into the block face and all the sections crumbled.  I
didn't seal the block after I was done and when I came back the next day
the entire tissue sample was shriveled and pulling away from the
paraffin.  I'm new, but in the few bone sections I've done I've never
had this happen!  Any ideas?

~Shelly



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