[Histonet] Tissue Shriveling in Paraffin

[Jack Ratliff]

Why not try resin embedding techniques? Less shrinkage than paraffin, more specimen/block stability in cutting, no damaging effects of decalcification and a many times you have a better and more clear morphological representation. I could help you to achieve this if interested, so feel free to contact me as needed (31-281-1975).

Jack

Jack Ratliff

Senior Histologist, Biomimetic Therapeutics, Inc.

Chair, Hard Tissue Committee - National Society for Histotechnology



On Jun 24, 2011, at 8:27 AM, "Michelle Aono" <aonomic <@t> auburn.edu> wrote:

> I was cutting some bone/joint tissue and noticed that the cartilaginous
> portion was concave/indented, instead of flush with the rest of the
> block surface.  Even as I continued to cut that portion always seemed a
> little sunken into the block face and all the sections crumbled.  I
> didn't seal the block after I was done and when I came back the next day
> the entire tissue sample was shriveled and pulling away from the
> paraffin.  I'm new, but in the few bone sections I've done I've never
> had this happen!  Any ideas?
> 
> ~Shelly
> 
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