[Histonet] sectioning frozen skin

[Rene J Buesa]

If you are able to cut them at 5 µm also, that will be fine.
René J.

--- On Tue, 6/21/11, Kalleberg, Kristopher <Kristopher.Kalleberg <@t> unilever.com> wrote:


From: Kalleberg, Kristopher <Kristopher.Kalleberg <@t> unilever.com>
Subject: [Histonet] sectioning frozen skin
To: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, June 21, 2011, 8:43 AM


Can anyone suggest what the optimal thickness of flash frozen skin
sections should be cut at?  I typically cut FFPE sections at 5 um,
should frozens be cut thinner or thicker?  These sections will be used
for H&E and routine IHC.  Thank you in advance.

Kris 
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet