[Histonet] RE: sectioning frozen skin

[Rittman, Barry R]

Kris
There is no simple answer to this as it depends.....
Thinner sections such as 4-5 microns offer better resolution with better localization of many substances within cells.
Thicker sections in the 7 to 10 micron range give better perspective of tissue and cell relationship and have more entire cells than the thinner sections.
If you are doing any sort of image analysis such as counting numbers, I think that you will find that the thinner you cut sections the greater the variability between sections due to compression, changes in cutting speed and probably also phases of the moon.
I would recommend that for your purposes you cut several sections at 5 microns, at 7 and at 10 microns and then compare these to find which best suits your needs.
Barry

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher
Sent: Tuesday, June 21, 2011 7:44 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] sectioning frozen skin

Can anyone suggest what the optimal thickness of flash frozen skin
sections should be cut at?  I typically cut FFPE sections at 5 um,
should frozens be cut thinner or thicker?  These sections will be used
for H&E and routine IHC.  Thank you in advance.
 
Kris 
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