[Histonet] sectioning frozen skin

[Patsy Ruegg]

I cut frozen sections about the same thickness as ffpe 4-6 microns typically
unless thicker is requested, we cut thru embryos and for those they want us
to cut 10 micron sections.

Regards,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
 

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kalleberg,
Kristopher
Sent: Tuesday, June 21, 2011 6:44 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] sectioning frozen skin

Can anyone suggest what the optimal thickness of flash frozen skin
sections should be cut at?  I typically cut FFPE sections at 5 um,
should frozens be cut thinner or thicker?  These sections will be used
for H&E and routine IHC.  Thank you in advance.
 
Kris 
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