[Histonet] Decal Solutions

[Patsy Ruegg]

I have used formic acid (5%) for decal for IHC for 20 years, it is slower
than HCL but much better for IHC, if you use a platform shaker to keep it
moving the exchange can be pretty good for bm bx 4-6 hours is what I would
start with.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gauch, Vicki
Sent: Monday, June 13, 2011 8:40 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Decal Solutions

Hi,
Does anyone know of any decal solutions that are compatible with IHC, ISH,
FISH other than EDTA and solutions containing HCL ?  We have heard that some
people use formic acid.  Also, what would be the decalcification times for
tissue such as a bone marrow bx using these other solutions ?  Any help
would be greatly appreciated...

Thanks,
Vicki Gauch
AMCH
Albany, NY



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