[Histonet] Decal Solutions

[Johnson, Nacaela]

We do 95% bone marrow specimens here.  We have perfected our ISH and IHC
using the Rapid-Cal Immuno from BBC.  Most bone marrows decal in 2.5
hours (8 gauge).  The decal does not diminish the iron stores either.
If you have questions about my procedures feel free to contact me. 


Thanks,
 
Nacaela Johnson, B.S. HTL (ASCP)CM
Histotechnologist
KCCC Pathology
12000 110th St., Ste. 400
Overland Park, KS 66210
Office:  913-234-0576
Fax:  913-433-7639
Email:  Nacaela.Johnson <@t> USOncology.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gauch,
Vicki
Sent: Monday, June 13, 2011 9:40 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Decal Solutions

Hi,
Does anyone know of any decal solutions that are compatible with IHC,
ISH, FISH other than EDTA and solutions containing HCL ?  We have heard
that some people use formic acid.  Also, what would be the
decalcification times for tissue such as a bone marrow bx using these
other solutions ?  Any help would be greatly appreciated...

Thanks,
Vicki Gauch
AMCH
Albany, NY



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