[Histonet] RE: Decal Solutions

[Tony Henwood]

Vicki,

We fix our trephines in 10% formal alcohol overnight and decal for 6 hours in formic acid (4% Formaldehyde, 33% Formic acid, 0.85% Sodium Chloride).

Excellent morphology and good antigen preservation.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gauch, Vicki
Sent: Tuesday, 14 June 2011 12:40 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Decal Solutions

Hi,
Does anyone know of any decal solutions that are compatible with IHC, ISH, FISH other than EDTA and solutions containing HCL ?  We have heard that some people use formic acid.  Also, what would be the decalcification times for tissue such as a bone marrow bx using these other solutions ?  Any help would be greatly appreciated...

Thanks,
Vicki Gauch
AMCH
Albany, NY



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