[Histonet] Re: Decal Soloutions
Thomas Huynh
thomas6093 <@t> yahoo.com
Mon Jun 13 15:08:24 CDT 2011
Hi Vicki
We use 10% Formic Acid to decal our bone marrow core biopsies( Microwave
decal-2.5 hours), then process as normal bx run on the processor.
Thomas Huynh
713-745-4759
________________________________
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To: histonet <@t> lists.utsouthwestern.edu
Sent: Mon, June 13, 2011 12:01:37 PM
Subject: Histonet Digest, Vol 91, Issue 17
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Today's Topics:
1. RE: Distribution of work for older generation of Histotechs
(Gomez, Milton)
2. general license question for Florida (Kim Tournear)
3. Formaldehyde in H2S's 12th RoC (Robert Richmond)
4. Decal Solutions (Gauch, Vicki)
5. Looking for replacement drive belt for Leica Autostainer XL
(taylor alan)
6. RE: Formaldehyde in H2S's 12th RoC (Bernice Frederick)
7. formalin in OR/L&D (Gale Limron)
8. Re: formalin in OR/L&D (Rene J Buesa)
9. RE: Looking for replacement drive belt for Leica
AutostainerXL (Sherwood, Margaret )
----------------------------------------------------------------------
Message: 1
Date: Sun, 12 Jun 2011 13:20:17 -0400
From: "Gomez, Milton" <Milton.Gomez <@t> nyumc.org>
Subject: [Histonet] RE: Distribution of work for older generation of
Histotechs
To: "Gomez, Milton" <Milton.Gomez <@t> nyumc.org>,
"'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4A53F9A1D7C2674FA4A6E650D703DDA5FCC7C7B7 <@t> MSGWSDCPMB07.nyumc.org>
Content-Type: text/plain; charset="us-ascii"
Thank you all for your input on this issue. I want to thank the older
histotechs in the field for their support, wisdom, courage and dedication.
Special thanks to those who are in bench positions and still cranking the
wheel! I hope that we are not getting rid of them but accommodating their
valued input for the improvement of our laboratories. I hope we can encourage
the younger histotechs to work with them as a team instead of competing with
them.
MG
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gomez, Milton
Sent: Sunday, June 05, 2011 1:09 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Distribution of work for older generation of Histotechs
Hello Histonetters,
Is the distribution of work different for older histotechs vs. younger
histotechs in your labs and why? Do they get special assignments or duties
because of their growing wisdom and seniority?
Thanks in advance,
MG
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Message: 2
Date: Sun, 12 Jun 2011 10:30:50 -0700 (PDT)
From: Kim Tournear <kim.tournear <@t> yahoo.com>
Subject: [Histonet] general license question for Florida
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <257237.74493.qm <@t> web120215.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi everyone,
I was asked to post the following question from a technician/Supervisor outside
of Florida:
If someone has their Florida State license for technician, and they are HT
certified (ASCP), but no BS degree, how many CE's (or is a BS required)
are needed in order to apply for the supervisor license? And is there an exam?
Thanks in advance for all replies......
~Kim~
OU ROCKS!!!!
~Don't be afraid your life will end,
be afraid it will never begin~
------------------------------
Message: 3
Date: Mon, 13 Jun 2011 10:11:26 -0400
From: Robert Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Formaldehyde in H2S's 12th RoC
To: "Histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BANLkTimM0hEFZZuwktUUXRupuHkvi1XcHQ <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
The US Department of Health and Human Services (sometimes
affectionately known as H2S) has released the 12th Report on
Carcinogens (RoC). The report lists formaldehyde as a definite human
carcinogen. The regulatory repercussions of this aren't immediately
clear, but I suspect pathologists will see increasing pressure to
eliminate formaldehyde fixation. As the snake oil peddlers come out of
the woodwork, it's going to become increasingly important to remember
that there is no substitute for formaldehyde that will not greatly
change the way pathologists and histologists work.
A not entirely satisfactory New York Times account is at:
http://www.nytimes.com/2011/06/11/health/11cancer.html?hp
The report itself can be obtained in pieces in PDF form at:
http://ntp.niehs.nih.gov/?objectid=72016262-BDB7-CEBA-FA60E922B18C2540
The 12th RoC reports repeat the mantra "studies show" at Joyce
Brothers length, but do not really make clear what the facts are. They
do offer these references about mortality among embalmers and
pathologists. Does anyone on the list have access to these papers?
Hauptmann M, Lubin JH, Stewart PA, Hayes RB, Blair A. 2003. Mortality
from lymphohematopoietic
malignancies among workers in formaldehyde industries. J Natl Cancer
Inst 95(21): 1615-1623.
Hauptmann M, Lubin JH, Stewart PA, Hayes RB, Blair A. 2004. Mortality
from solid cancers among workers
in formaldehyde industries. Am J Epidemiol 159(12): 1117-1130.
Hauptmann M, Stewart PA, Lubin JH, Beane Freeman LE, Hornung RW,
Herrick RF, et al. 2009. Mortality
from lymphohematopoietic malignancies and brain cancer among embalmers
exposed to formaldehyde.
J Natl Cancer Inst 101(24): 1696-1708.
Posting this both to PATHO-L and to Histonet.
Bob Richmond
Samurai Pathologist
Knoxville TN
------------------------------
Message: 4
Date: Mon, 13 Jun 2011 10:40:08 -0400
From: "Gauch, Vicki" <GauchV <@t> mail.amc.edu>
Subject: [Histonet] Decal Solutions
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C5E841FC4B5BA94987B9E68B5396A9AF597CF53B68 <@t> MS-EXCH-CCR02.AMCNT.AMC.EDU>
Content-Type: text/plain; charset="us-ascii"
Hi,
Does anyone know of any decal solutions that are compatible with IHC, ISH, FISH
other than EDTA and solutions containing HCL ? We have heard that some people
use formic acid. Also, what would be the decalcification times for tissue such
as a bone marrow bx using these other solutions ? Any help would be greatly
appreciated...
Thanks,
Vicki Gauch
AMCH
Albany, NY
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Message: 5
Date: Mon, 13 Jun 2011 15:58:04 +0100
From: taylor alan <aj.taylor <@t> blueyonder.co.uk>
Subject: [Histonet] Looking for replacement drive belt for Leica
Autostainer XL
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <BANLkTin=qeB7-4THeHXuTxopw_vdX+mceg <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hello All.
We have recently aquired an 'oldish' AutostainerXL. It worked perfectly up
until the weekend, then the drive belt that lifts the robotic arm decided to
break! We are quite keen to find a replacement belt
as soon as possible.
I am asking if any of you Autostainer users have sources for replacement
drive belts other than Leica, as it is apparently no longer listed for
replacement parts on their web pages.
Any help and assistance would be gratefully received.
Regards
Alan
Alan Taylor
Microtechnical Services
71 Sweetbrier Lane
Heavitree
Exeter. Devon. EX1 3AJ
U.K.
Tel +44 (0) 1392 660132
Fax +44 (0) 1392 209990
------------------------------
Message: 6
Date: Mon, 13 Jun 2011 10:05:43 -0500
From: "Bernice Frederick" <b-frederick <@t> northwestern.edu>
Subject: RE: [Histonet] Formaldehyde in H2S's 12th RoC
To: "'Robert Richmond'" <rsrichmond <@t> gmail.com>,
"'Histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <003101cc29db$5d90a940$18b1fbc0$@northwestern.edu>
Content-Type: text/plain; charset="us-ascii"
Well we still use Hematoxylin powder, Xylene ,Oil Red O, Beibrich scarlet
and Congo red and we haven't been stopped yet.
I'd think embalmer would be well-fixed and preserved ,rather than have a
high mortality rate. Have the proven it's from formaldehyde? Whom knows what
the get from a dead body?
Bernice
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert
Richmond
Sent: Monday, June 13, 2011 9:11 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Formaldehyde in H2S's 12th RoC
The US Department of Health and Human Services (sometimes affectionately
known as H2S) has released the 12th Report on Carcinogens (RoC). The report
lists formaldehyde as a definite human carcinogen. The regulatory
repercussions of this aren't immediately clear, but I suspect pathologists
will see increasing pressure to eliminate formaldehyde fixation. As the
snake oil peddlers come out of the woodwork, it's going to become
increasingly important to remember that there is no substitute for
formaldehyde that will not greatly change the way pathologists and
histologists work.
A not entirely satisfactory New York Times account is at:
http://www.nytimes.com/2011/06/11/health/11cancer.html?hp
The report itself can be obtained in pieces in PDF form at:
http://ntp.niehs.nih.gov/?objectid=72016262-BDB7-CEBA-FA60E922B18C2540
The 12th RoC reports repeat the mantra "studies show" at Joyce Brothers
length, but do not really make clear what the facts are. They do offer these
references about mortality among embalmers and pathologists. Does anyone on
the list have access to these papers?
Hauptmann M, Lubin JH, Stewart PA, Hayes RB, Blair A. 2003. Mortality from
lymphohematopoietic malignancies among workers in formaldehyde industries. J
Natl Cancer Inst 95(21): 1615-1623.
Hauptmann M, Lubin JH, Stewart PA, Hayes RB, Blair A. 2004. Mortality from
solid cancers among workers in formaldehyde industries. Am J Epidemiol
159(12): 1117-1130.
Hauptmann M, Stewart PA, Lubin JH, Beane Freeman LE, Hornung RW, Herrick RF,
et al. 2009. Mortality from lymphohematopoietic malignancies and brain
cancer among embalmers exposed to formaldehyde.
J Natl Cancer Inst 101(24): 1696-1708.
Posting this both to PATHO-L and to Histonet.
Bob Richmond
Samurai Pathologist
Knoxville TN
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
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------------------------------
Message: 7
Date: Mon, 13 Jun 2011 11:16:00 -0400
From: Gale Limron <GaleL <@t> unionhospital.org>
Subject: [Histonet] formalin in OR/L&D
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D7B0000361AAAF4A8895E5918634850A04C85562F9 <@t> uhexg-3.unionhospital.org>
Content-Type: text/plain; charset="us-ascii"
Hi,
I know this topic has been discussed time-to-time but I was just approached with
questions regarding the formalin cubes that are supplied to our OR and L&D
departments.
OR has a small room where 2.5 gal. cubes are kept under a hood. They set on a
metal stand in a plastic tray. They would like to keep a layer of
spill-absorbing material in the plastic tray to neutralize any drips or spills.
Is this advisable?
L&D has 5 gal. formalin cubes that are kept beside a sink in an area with no
hood. If there are any drips or leaks they are washed down the drain. Any
opinions on this system of storage?
I would appreciate hearing how other places handle these types of storage
issues.
Thank you,
Gale
Gale Limron CT, HT (ASCP)
Histology Supervisor
Union Hospital
659 Boulevard
Dover, Ohio 44622
330-343-3311 ext 2562
This e-mail is intended only for the person or entity to which it is addressed
and may contain information that is privileged, confidential or otherwise
protected from disclosure. Dissemination, distribution or copying of this e-mail
or the information herein by anyone other than the intended recipient, or an
employee or agent responsible for delivering the message to the intended
recipient, is prohibited. If you received this message in error, please delete
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------------------------------
Message: 8
Date: Mon, 13 Jun 2011 08:51:49 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] formalin in OR/L&D
To: Gale Limron <GaleL <@t> unionhospital.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <343330.83882.qm <@t> web65702.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Keeping formalin under a hood over a spill control pad is an acceptable
practice, but having a larger formalin container out of a hood and dripping to a
drain is totally unacceptable, more because it is an open container than because
of a small dripping to the sewer.
René J.
From: Gale Limron <GaleL <@t> unionhospital.org>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, June 13, 2011 11:16 AM
Subject: [Histonet] formalin in OR/L&D
Hi,
I know this topic has been discussed time-to-time but I was just approached with
questions regarding the formalin cubes that are supplied to our OR and L&D
departments.
OR has a small room where 2.5 gal. cubes are kept under a hood. They set on a
metal stand in a plastic tray. They would like to keep a layer of
spill-absorbing material in the plastic tray to neutralize any drips or spills.
Is this advisable?
L&D has 5 gal. formalin cubes that are kept beside a sink in an area with no
hood. If there are any drips or leaks they are washed down the drain. Any
opinions on this system of storage?
I would appreciate hearing how other places handle these types of storage
issues.
Thank you,
Gale
Gale Limron CT, HT (ASCP)
Histology Supervisor
Union Hospital
659 Boulevard
Dover, Ohio 44622
330-343-3311 ext 2562
This e-mail is intended only for the person or entity to which it is addressed
and may contain information that is privileged, confidential or otherwise
protected from disclosure. Dissemination, distribution or copying of this e-mail
or the information herein by anyone other than the intended recipient, or an
employee or agent responsible for delivering the message to the intended
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delivery.
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------------------------------
Message: 9
Date: Mon, 13 Jun 2011 12:16:41 -0400
From: "Sherwood, Margaret " <MSHERWOOD <@t> PARTNERS.ORG>
Subject: RE: [Histonet] Looking for replacement drive belt for Leica
AutostainerXL
To: "taylor alan" <aj.taylor <@t> blueyonder.co.uk>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<073AE2BEA1C2BA4A8837AB6C4B943D9708DB57F1 <@t> PHSXMB30.partners.org>
Content-Type: text/plain; charset="us-ascii"
First, I would check with a Leica rep and see if he can help you. We have
ordered many replacement parts for our Autostainer XL (refurbished).
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of taylor alan
Sent: Monday, June 13, 2011 10:58 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Looking for replacement drive belt for Leica AutostainerXL
Hello All.
We have recently aquired an 'oldish' AutostainerXL. It worked perfectly up
until the weekend, then the drive belt that lifts the robotic arm decided to
break! We are quite keen to find a replacement belt
as soon as possible.
I am asking if any of you Autostainer users have sources for replacement
drive belts other than Leica, as it is apparently no longer listed for
replacement parts on their web pages.
Any help and assistance would be gratefully received.
Regards
Alan
Alan Taylor
Microtechnical Services
71 Sweetbrier Lane
Heavitree
Exeter. Devon. EX1 3AJ
U.K.
Tel +44 (0) 1392 660132
Fax +44 (0) 1392 209990
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