AW: [Histonet] Decal Soloutions

[Gudrun Lang]

We fix overnight in NBF and decal for 8 hours in "Calrite" (10% Formicacid,
5% Formaldehyd).
For FISH we had issues to demonstrate DNA-loci. CISH for kappa- and
lambda-mRNA works.

We tried a variant with 2 hours acid decal and 6 hours EDTA+Ultrasound.
Morphology in HE is ok, but IHC suffers in comparison to acid alone.
But with this short acid decal FISH has worked.
Decision if acid or combined decal is still not done. Few cases that require
FISH versus everyday IHC.

Bye Gudrun