[Histonet] Slide Bright Question

[DKBoyd <@t> chs.net]

Christopher
I use Americlear and have not had a staining problem.  Your last alcohols 
must be absolute and as stated previously your mounting media must be 
compatible.  I do not, however, use Americlear on the processor as we did 
have processing problems there.   We use ProPar from Anatech on the 
processor. 
With the expense and carcinogen factor of Xylene, this set up works great 
for us. 
As with any clearing agent, water contamination , produces leching eosin 
as one of many problems.



Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkboyd <@t> chs.net







Christopher Conlisk <cconlisk <@t> kcskincenter.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
06/03/2011 02:17 PM

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Subject
[Histonet] Slide Bright Question






Hello Everyone,
    I have worked in labs that use Xylene my entire career and I just
started at a Lab That only uses a Xylene Substitute "Slide-Bright". I am
having problems with the H&E. After staining and coverslipping (The slides
look fine innitially), Then about 5-10 minutes after coverslipping the 
Eosin
starts bleeding out all around the tissue. I have asked several of my
Histotech Friends that are old timers and they say that Xylene Substitutes
are awful at deparrifinization and awful at clearing. They told me that 
the
alcohol isnt getting thoroughly cleared in the "Slide Brite" and then it 
is
eventually leeching out after coverslipping??? Is this true and does 
anyone
have any guidance for this issue? We also run MOHS slides on the same
stainer and I keep all the reagents clean as a whistle. I really hate 
Xylene
Substitute's....

Thanks

C.S. Conlisk HT(ASCP), PBT(ASCP)
Kansas City Skin and Cancer Center
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