[Histonet] hand staining, bench top manual no automation
madary <@t> verizon.net
madary <@t> verizon.net
Fri Jun 3 14:15:21 CDT 2011
When I was in the field of histology and performing IHC manually, I
fo usage, eas reuse). Slide a waiting for the next job,any
Nick(Rocky) Madary, HT/HTL(ASCP)QIHC
Jun 3, 2011 12:01:25 P
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< 1. RE: Counter top immunos (Smith, Allen)
2. l 3. RE: [IHCRG] lam Chlipala)
4. RE: Counter top immu 5. Alternatives to Cytospin? (JR R)
6. RE: Counter top 7. RE: Alternatives to Cytospin? (Tony Henwood)
8. 9. Working V 10. Re: Bone Marrow H&E Proto 11. Re: nuclear haziness (Jean Brinker)
12. prici 13. Counter top immunos (Ji 14. Submission of tonsils to pathology (Richard Cartun)
15. 16. Re: Submission of tonsil 17. Re: Processing Mouse Pancreas (Rene J 18. RE: Submission of tonsils to pathology (Horn, Hazel V)
19. ------ ----------------------------------------------------------------
Mes Date: Thu, 2 Jun 2011 18:05:30 +0000
From: "Smith, Allen"
Subject: [Histonet] RE: Counter top immunos
To: Cc: "histonet <@t> lists.utsout Message-ID:
I like the Vector ABC the Nova Red chromogen. Mouse better results than rabbit or goat prim -----Original Message-----
From: histonet-bounces <@t> lists.ut [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Beha Marshall, Kimberly K
Sent: Thursday, June 02, 2011 9:57 AM
To: Subject: [Histonet] Counter top immuno Hello Histo folks
Hope everyone is having a great week....I info
on immuno kits for staining just by hand, l started
in this field. We are a small lab in Alask immunos are
sent out of state, We are looking to do som to
save money, but don't really need a stainer. S kits
that are better than others? Is there anyone that d this way
that would give some much needed advise???
A Kimberly
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Message: Date: Thu, 2 Jun 2011 13:05:28 -0500
From: "Perry, Margaret"
Subject: [Histonet] laminin and collagen4 in cat To: "ihcrg <@t> googlegroups.com" ,
"histo Mes Content-Type: text/plain; charset="us-ascii"
We want to use some spindle cell tumors. sheath tumors reportedly show lam membranes vs. Fibrosarcoma which does not. We will work in both cat and dog. Is there anyone who ha success with these? Do you have any recommendations for these
antibod Margaret Perry HT(ASCP)
Dept of Veterina Box 2175
South Dakota State Univer Brookings SD 57007
605-688-5638
- Message: 3
Date: Thu, 2 Jun 2011 12 From: "Liz Chlipala"
Subject: [Hist dog
To: "Perry, Marga ,
Message-ID:
Content-Type: text/plain; charset="us Margaret
We have used the Collagen IV listed feline, for
laminin we have not used the antibod feline but
the spec sheet states it works on m Good Luck
Liz
Col SouternBio
0340-01
UNLB
Goat
Laminin
abcam
ab11575
Rabbit
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
PO Box 18592
Boulder, Colorado 80308< office (303) 682-3949
fax (303) 682-9060
www.premierl Ship to Address:
1567 Skyway Drive, Un Longmont, Colorado 80504
-----Original Message-----
F Behalf
Of Sent: Thursday, June 02, 2011 12:05 PM
To: ihcrg <@t> goo Subject: [IHCRG] lamini We want to use laminin and Co some
spindle cell tumors. Specifical sheath
tumors reportedly show laminin and vs.
Fibrosarcoma which does not. We need both
cat and dog. Is there anyone who has you
have any recommendations for these antibo Margaret Perry HT(ASCP)
Dept of Veter Box 2175
South Dakota State Uni Brookings SD 57007
605-688-5638
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To conta histo <@t> nsh.org or cal ------------------------------
Messag Date: Thu, 2 Jun 2011 13:50:43 -0500
From: hymclab
Subject: [Histonet] RE: Counter top immunos
T Cc: "histonet <@t> lists.utsouthwestern.edu"
Message-ID:
Content-Type: text/pla We do ours manually also (about 20 differen a combo of BioGenex and Dako. I like both set ups, bu more expensive to perform than BioGenex. I like that you can buy the in dividual components from BioGenex instead of a whole ket
from DAKO.< Dawn
-----Original Message-----
From: histonet-bounces <@t> lis [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Smith, Allen
Sent: Thursday, June 02, 2011 1:06 PM
To: Mar Cc: histonet <@t> lists.utsouthwestern.edu
Subject: [His I like the Vector ABC horseradish per with the Nova Red chromogen. Mouse primary antibod give me better results than rabbit or goat primaries.
-- From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Marshall, Sent: Thursday, June 02, 2011 9:57 AM
To: histonet <@t> lists.u Subject: [Histonet] Counter top immunos
Hello H Hope everyone is having a great week....I am writing to g info on immuno kits for staining just by hand, like back when I
fir of our immun of them here and try to save money, but don't really need a stainer. So are there any kits
t this way that Again thanks in advance for a Kimberly
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attachme ----------------------- Message: 5
Date: Thu, 2 Jun 2011 16:54:29 -0700
From: Subject: [Histonet] Alternatives to Cyto To:
Message-ID:
Content-Type: text/plain; charset=" I will be collecting bronchoalveolar lavage fluid (B mice and want to do do a diff-quick stain on the fluid to charact erize the leukocyte population.
My colleagues usually use a cytospin slide but I'll be at a lab that doesn't have t looking for alternatives.
Could I just put a dro dry it? Or treat the BALF like a Alternatively, could I fix the cells in...ethanol mayb cytospin them the next day when I get back to my lab?
Any Jerry Ricks
Research ScientistUniversity of Washington
Department of Pathology
-- Message: 6
Date: Thu, 2 Jun 2011 17: From: JR R
Subject: [Histonet] To:
M Content-Type: t For mouse and rabbit primary a ImmPRESS kits. They save time. For HRP substrat too dull to me. I have had good results with AEC and also Vector ImmPACT DAB.
Jerry Ricks
Research Scientist
Unive Department of Pathology
-----Original Message From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-b ounces <@t> lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K
Sent: To: histonet <@t> lists.utsouthwestern.eduSubject: [Histonet] Counter
top immunos
Hello Histo folks
H info on imm first started in this of our immunos are sent out of of them here and try to
save money, but that are better than this way that would give some mu Again thanks in advance for all the imput...
Kimberly
------------------------------
Message: 7
Da From: Tony Henwood
Subject: RE: [Histonet] Alternatives to Cytospin?
To: "'JR R'" ,
"histonet <@t> lists.utsouthwestern.edu"
Message-ID: <6D6BD1DE8A5571489398B392A38 A7157188590A3 <@t> xmdb02.nch.kids>
Content-Type: text/plain; charset="u Jerry,
Firstly, I would not recommend fixing in etha shrink and Romanowsky staining will be compromised (th stain too darkly since they are smaller).
I would use a cent a blood film or if you do no a drop of the BALF on the slide and ei suggested (if cell count is low) or treat it like blood looks quite opaque - lots of cells/ & other junk).
Regar Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fa the children's hospital at westmead
Cnr Hawkesbury Locked Bag 4001, Westmead NSW 2145, -----Original Message-----
From: histonet-bounces <@t> lis [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On R
Sent: Friday, 3 June 2011 9:54 AM
To: histonet <@t> lists. Subject: [Histonet] Alternatives to Cytospin?
I will be collecting bronchoalveolar lavage fluid (BALF) from mice
and the leukocyte My colleagues usually use a cytospin to concentrate cel slide but I'll be at a lab that doesn't have the equipment so I'm looking for alternatives.
Could I just put a drop of the BALF onto dry it? Or treat the BALF like a blood smear?
Alternatively, could I fix the cells in...ethanol maybe and then
cytospin Any ideas will be much Jerry Ricks
Research Scientist
University of Was Department of Pathology
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Message: 8
Date: From: "histotech <@t> imagesbyhopper.com"
Subject: Re: [Histonet] RE: printed requisit To: "Horn, Hazel V"
Cc: "histonet <@t> lists Message-ID: <92F4768F-F009-41BB-A10C-02B799A4F400 <@t> imagesbyhopper.com>
Content- We currently use paper reqs, bu paperless. I am not sure I am going t staff will enter the "req" in the computer, we the specimen and enter into our AP system.
Sent from my iPhone
On Jun 2, 2011, at 8:18 AM, "Horn, Hazel V" wrote > We still use paper reqs.
>
> Hazel Horn
> > Supervisor of Autopsy/Histology/Transcrip > Arkansas Children's Hospital
> 1 Children's Way Slot 820 > Little Rock, AR 72202
>
> phone 501.364.4240
> >
> visit us on the web at: www.archildrens.o >
>
> -----Original Message-----
> From: histo [mailto:histonet-bounces <@t> lists.utsouth Rathborne, Toni
> Sent: Wednesday, June 01, > To: histonet <@t> lists.utsouthwestern.edu
> Subject >
>
> Does everyone s surgical specimens? How many of room? Our OR has a shared printer for all but would like to have a printer for each OR suite department is saying there would be problems and has suggested tha t we go paperless. I see many problems arising from this. If anyone
is doin you accomplished >
>
> Toni
>
> Regards,
> Toni > Pathology Supervisor
> Somerset Medical Center
& > Somerville, NJ 08876
>
>
> >
> CONFIDENTIALITY NOTICE
> This message and any inclu Medical Center
> and are intended o contained in this
> message is confidential,
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addressee, please > promptly delete this message and notify the sender of the
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------------------------------
Date: Thu, 2 Jun 2011 20:33:45 -0700
From: Marc Shaeffer < Subject: [Histonet] Working Ventana Probe ProtocolsTo:
histonet <@t> lists.utsouthwestern.edu
Message-ID: <20110602233345.O Content-Type: text/plain; charset=ut > Working Ventana Probe Protocols!
>
> Heres a Ventana Probes
> > Kappa Probe
>
> -Depar 16 min
& > -ISH (Kappa) Probe 4min
> > -Hybe 1 hour
> - 3 stringenc > -Blue detection for 20 minutes
> > Lambda and EBER Probes
>
> Sam minutes. Everything > the same.
> I also learned that there is no need probe as they
> are predilutes and ready to sell is just
> for using other probe > Hopefully this helps everyone running these probes that nee starting point!
I am not affiliated with ventana.
Marc S ------------------------------
Message: 10
Date: Thu, 2 Jun 2011 21:27:38 -0700 (PDT)
From: Dawud AlYasa
Subject: Re: [Histonet] Bone Marrow H&E Protocols
To Message-ID: <726474.45849.qm <@t> web3 Content-Type: text/plain; charset=iso-8859 What's up Tena!
Long time. The bone marrows will be fixe Dawud
------------------------------
Message: 11 Date: Fri, 3 Jun 2011 02:57:55 -0700 (PDT)
From: Jean Brinker
Subject: Re: [Histonet] nuclear haziness
To: Rene J Bue "histonet <@t> lists.utsouthwestern.edu"
Message-ID: <50710.84775.qm <@t> web180304.mail Content-Type: text/plain; charset=iso-8859-1
Thanks for your response. I had that thought as well, and the techs
have < drained from slide they are placed in the oven. They check them again before stai ning.
________________________________
From: Ren To: Jean Brinker ; "his Subject: Re: [Histonet] nuclear hazin Make sure that ALL your slides are properly dried in the ove that NONE has
any remnant of water underneath before going into t dry.
René J.
From: Jean Brinker
To: histonet <@t> lists.utsouthwestern.edu
Sent: Thursday, June 2, 2011 Subject: [Histonet] nuclear haziness
We are having an is in our small
urology lab and not ot within the same block. Another scenario is that we sometimes s it occur only
in one small area of a core and the remaining tissue i looks
beautiful. It can occur throughout a case (12 blocks) all. We have
checked for obvious problems, i.e. reagent conta processor and
stainer, ruled out temperature issue to no avail. I
would appreciate any input/s alleviate this
problem.
Thank you Jean Brinker
St. Louis Urological Surgeons
St. Peters, MO
_______________________________________________
Histonet mailing listHistonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mail ------------------------------
Message: Date: Fri, 3 Jun 2011 06:09:14 -0400
From: "Della Speranza, Vinni Subject: [Histonet] pricing for EM on kidney
To: Message-ID:
Content-Type: text/plain; charset="us-ascii"
I've been from labs that acce your fee for EM only and not a c In addition to pricing for kidney samples pr gluteraldehyde, I'll also need to know if your pricing is differe nt if you are asked to work from a paraffin block.
thank you,
Vinnie Della Speranza, MS, HTL(ASCP)
Manager for Anatomic Pathology Ser Medical University of South Carolina
165 Ashley Avenue Suite 30 Charleston, SC 29425
ph (843) 792-6353
fax (843) 792-8974
___ ---------- Message: 13
Date: Fri, 3 Jun 2011 12:42:32 + From: "Jim Reilly"
Subject: [Histon To:
Messa Content-Type: text/plain; charset="us-ascii"
Hello We do all our immunos manually and the nearest thing to a use is
the ImmPRESS Peroxidase Polymer Detection System or VECTAS ABC
System with ImmPACT DAB Peroxidase Substrate all from Vector
Laboratories. The majority of our Ab's are used on human tissue and
the
first choice for primary Ab's for us is mouse anti-human raised in
hors Cheers
Jim Reilly
Senior Histology Technician
Instit Medical,
Veterinary Place
Glasgow
Tel: +44 141 330 7573
----------------------- Message: 14
Date: Fri, 03 Jun 2011 11:55:21 -0400
From Subject: [Histonet] Submission To: "Histonet"
Message-ID: <4DE8CBA8.7400.0077.1 <@t> harthosp.org>
Content-Typ What is your policy on the submiss examination (gross or microscopic)? Thank y Richard
Richard W. Cartun, MS, PhD
Director, Histology Director, Biospecimen Collection Programs
Assi Hartford Hospital
80 Seymour Stree Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
< ------------------------------
Message: 15
Dat From: "Stoll, Kathryn"
Subject: [Histonet] Processing Mouse Pancreas
To: "histonet <@t> lists.u Message-ID:
<110E7925E2B91945A9B79EDFD0DC2B34E2A188859B <@t> MCWMBX2.mcwcorp.net>
Happy Friday Everyone Does anyone have a protocol for processing mouse pancreas? We have
a V I am not familiar with the processing any animal tissue and do not
Thanks in advance.
Kathryn St Depatment of Pathology
Medical College of Wisconsin
Milwaukee WI 53226
414.805.1525
kstoll <@t> mcw.ed ------------------------------
Message: 16
Date: From: Rene J Buesa
Subject: Re: [Histonet] Submission of tonsils to pathology
To: H Message-ID: <231345.32485.qm <@t> web65716.mail.ac4.yahoo.com&g Content-Type: text/plain; charset=iso-8859-1
Labelled "gross René J.
--- On Fri, 6/3/11, Richard Cartun wrote:
From: Richard Cartun < tonsils to pathology
To: "Histonet" Date: Friday, June 3, 2011, 11:55 A What is your policy on the submission of tonsils to pathology examination (gross or microscopic)? Thank you.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology Director, Biospecimen Collection Programs
Assistant Director, Anatom Hartford Hospital
80 Seymour Street
Hartford, CT (860) 545-1596 Office
(860) 545-2204 Fax
_____ Histonet mailing list
Hist http://lists.utsouthwestern.edu/mailman/li ------------------------------
Message: 1 Date: Fri, 3 Jun 2011 09:12:18 -0700 (PDT)
From: Rene J Buesa
Subject: Re: [Histonet] Processing Mouse Pancreas
To: , Message-ID: <722585.84511.qm <@t> web65712. Content-Type: text/plain; charset=iso-8859-1
Develop a good general protocol for animal tissue. You cannot have
"dedicated" protocols for every type of tissue.
René J.
--- From: Sto Subject: [Histonet] Processing Mouse Pancre To: "histonet <@t> lists.utsouthwestern.edu"
Date: Friday, June 3, 2011, 12:03 PM
Happy Friday Ever Does anyone have a protocol for processing mouse pancreas? W have a VIP6.
I am not familiar with the processing any animal tissue a not know if it is similar to human.
Thanks in advance.
Kathryn Stoll, HT(ASCP)
Depatment of Pathology
Medical College 9200 W Wisconsin Ave
Milwaukee WI 53226
414.805.1525< _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists ------------------ Message: 18
Date: Fri, 3 Jun 2011 11:13:44 -0500
Subject: RE: [Histonet] S To: "'Rene J Buesa'" , Histonet
, Richard Cartun
Message-ID:
<25A4DE08332B19499904459F00AAACB719 Content-Type: text/plain; charset="i Generally all are gross only. There are some exceptions d on patient history or if one is significantly larger than the othe r the pathologists have the discretion to do a microscopic.
Hazel H Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcr Arkansas Children's Hospital
1 Children's Way Slot 820
Litt phone 501.364.4240
fax 501.364.3155
visi -----Original Message--- From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-boun Rene J Buesa
Sent: Friday, Ju To: Histonet; Richard Cartun
Subject: Re: [Histo Labelled "gross only".
Re --- On Fri, 6/3/11, Richard Cartun From: Richard Cartun
Subject: [ To: "Histonet"
Date: Friday, June 3, 2011, 11:55 AM
examinati Richard
Richard W Director, Histology & Immunopathology
Director, Assistant Director, Anatomic Pathology< 80 Seymour Street
Hartford, CT 06102
(8 (860) 545-2204 Fax
__________________ Histonet mailing list
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The inf confidential
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< Date: Fri, 03 Jun 2011 12:40:47 -0400
From: "Richard C Subject: [Histonet] Submission of tonsils To: "Histonet"
Content-Type: text I apologize; I should have stated my ques Children's Hospital wants to stop sending tonsils to unless the surgeon requests pathological examination. An article published in Otolaryngology-Head and Neck Surgery (Nelson et al.)
concluded cost-effective use of limi of unsuspected pathology were identi specimen evaluation (n=4186) and positive pathologi on microscopic analysis (n=1066) were only identified in post- transplant patients."
Have any of you exempted tonsils from submissi not, maybe we should.
Richard
Richard W. Director, Histology & Immunopathology
Director, B Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545- (860) 545-2204 Fax
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