[Histonet] Slide Bright Question

[Rene J Buesa]

General advise:
To dewax sections before staining I used 2% aqueous solution of dishwasher soap at 90ºC (2 changes) with admirable results. You go directly to water → stain.
After staining, I just dried the stained sections in an oven at 60ºC for 5 minutes → coverslip.
Try it.
René J.

--- On Fri, 6/3/11, Debra Siena <DSiena <@t> statlab.com> wrote:


From: Debra Siena <DSiena <@t> statlab.com>
Subject: RE: [Histonet] Slide Bright Question
To: "Christopher Conlisk" <cconlisk <@t> kcskincenter.com>, "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Friday, June 3, 2011, 2:31 PM


Hi Christopher,

There are differences between how Xylene substitutes works and Xylene.  First of all, Xylene subs can be a bit slower to deparaffinize, so you may need to increase the time or # or stations when deparaffinizing.  Also, Xylene subs don't tolerate water so your last alcohol must be 100% or else you will see the eosin bleed out of the sections.  The other thing about Xylene substitutes is that the mounting media must be compatible with the substitute.   If I can help in any other way, please let me know.  thanks

Debbie Siena HT(ASCP)QIHC
Technical Manager | StatLab Medical Products
Direct: 972-436-1010  x229 



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Christopher Conlisk
Sent: Friday, June 03, 2011 1:18 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Slide Bright Question

Hello Everyone,
    I have worked in labs that use Xylene my entire career and I just
started at a Lab That only uses a Xylene Substitute "Slide-Bright". I am
having problems with the H&E. After staining and coverslipping (The slides
look fine innitially), Then about 5-10 minutes after coverslipping the Eosin
starts bleeding out all around the tissue. I have asked several of my
Histotech Friends that are old timers and they say that Xylene Substitutes
are awful at deparrifinization and awful at clearing. They told me that the
alcohol isnt getting thoroughly cleared in the "Slide Brite" and then it is
eventually leeching out after coverslipping??? Is this true and does anyone
have any guidance for this issue? We also run MOHS slides on the same
stainer and I keep all the reagents clean as a whistle. I really hate Xylene
Substitute's....

Thanks

C.S. Conlisk HT(ASCP), PBT(ASCP)
Kansas City Skin and Cancer Center
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