[Histonet] RE: Counter top Immunos...

[JR R]

For mouse and rabbit primary antibodies I like the Vector ImmPRESS kits.  They save time.  For HRP substrates Nova Red looks too dull to me.  I have had good results with AEC and also with Vector ImmPACT DAB.

Jerry Ricks
Research Scientist
University of Washington
Department of Pathology

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K
Sent: Thursday, June 02, 2011 9:57 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Counter top immunos
 
Hello Histo folks
 
  Hope everyone is having a great week....I am writing to get some info on immuno kits for staining just by hand, like back when I first started in this field.  We are a small lab in Alaska and all of our immunos are sent out of state,  We are looking to do some of them here and try to
save money, but don't really need a stainer.   So are there any kits
that are better than others?  Is there anyone that does immunos this way that would give some much needed advise???
 
  Again thanks in advance for all the imput...
 
Kimberly