[Histonet] Working Ventana Probe Protocols

[Marc Shaeffer]

> Working Ventana Probe Protocols!  
> 
> Heres a good starting point when using the Kappa/Lambda/EBER Ventana Probes
> on the Ultra:

> Kappa Probe 
> 
> -Depar 16  min
> - Enzyme Protease 2 for 4 min,
> -ISH (Kappa) Probe 4min
> -Denature @ 85 degrees for 12min
> -Hybe 1 hour
> - 3 stringency washes for 8 minutes each
> -Blue detection  for 20 minutes
> -counterstain 4 min

> Lambda and EBER Probes
> 
> Same as Kappa but use the ISH  (Lambda and or EBER) Probe 4 minutes. Everything else is
> the same.
 
> I also learned that there is no need to dilute out Ventana's probe as they
> are predilutes and ready to use. The probe diluent that they sell is just
> for using other probes. 
 
> Hopefully this helps everyone running these probes that need a starting point! 

I am not affiliated with ventana.

Marc Shaeffer