[Histonet] RE: Counter top immunos

Smith, Allen asmith <@t> mail.barry.edu
Thu Jun 2 13:05:30 CDT 2011


I like the Vector ABC horseradish peroxidase kits, especially with the Nova Red chromogen.  Mouse primary antibodies usually give me better results than rabbit or goat primaries.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K
Sent: Thursday, June 02, 2011 9:57 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Counter top immunos

Hello Histo folks
 
  Hope everyone is having a great week....I am writing to get some info
on immuno kits for staining just by hand, like back when I first started
in this field.  We are a small lab in Alaska and all of our immunos are
sent out of state,  We are looking to do some of them here and try to
save money, but don't really need a stainer.   So are there any kits
that are better than others?  Is there anyone that does immunos this way
that would give some much needed advise???
 
  Again thanks in advance for all the imput...
 
Kimberly
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