[Histonet] RE: Counter top immunos

hymclab hymclab.hymclab <@t> ministryhealth.org
Thu Jun 2 13:50:43 CDT 2011


We do ours manually also (about 20 different antibodies).  We use a combo of BioGenex and Dako.  I like both set ups, but Dako is more expensive to perform than BioGenex.  I like that you can buy the in dividual components from BioGenex instead of a whole ket from DAKO.

Dawn

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Smith, Allen
Sent: Thursday, June 02, 2011 1:06 PM
To: Marshall, Kimberly K
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Counter top immunos

I like the Vector ABC horseradish peroxidase kits, especially with the Nova Red chromogen.  Mouse primary antibodies usually give me better results than rabbit or goat primaries.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K
Sent: Thursday, June 02, 2011 9:57 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Counter top immunos

Hello Histo folks

  Hope everyone is having a great week....I am writing to get some info on immuno kits for staining just by hand, like back when I first started in this field.  We are a small lab in Alaska and all of our immunos are sent out of state,  We are looking to do some of them here and try to
save money, but don't really need a stainer.   So are there any kits
that are better than others?  Is there anyone that does immunos this way that would give some much needed advise???

  Again thanks in advance for all the imput...

Kimberly
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