[Histonet] blurry tissue

[Rene J Buesa]

You just pointed out to the most likely causes: happening during staining, high humidity and water in the xylene. Try to take care of these issues and you will resolve the problem.
René J.

--- On Mon, 7/25/11, Carol Bryant <cbrya <@t> lexclin.com> wrote:


From: Carol Bryant <cbrya <@t> lexclin.com>
Subject: [Histonet] blurry tissue
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Monday, July 25, 2011, 1:42 PM


I would like some thoughts on how to resolve some "blurry" looking tissue.  We have had occasional tissue that looks blurry and not crisp for several weeks now.  It is not all the cases only random tissues.  The tissue is not on the same tissue processor either. We have 2 processors.  The latest cases were a breast, some skins, and a prostate.  I am not certain if this is happening on the tissue processor or in the stainer.  It has been very humid in our lab so I have started running a dehumidifier in case there is water in the xylene.  It is so hit and miss that I am puzzled.   Any suggestions would be greatly appreciated.
Thank you in advance for your thoughts.

Carol Bryant, CT (ASCP)
Cytology/Histology Manager
Pathology Services
Lexington Clinic
Phone (859) 258-4082
Fax (859) 258-4081
cbrya <@t> lexclin.com



NOTICE OF CONFIDENTIALITY

This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations.  If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited.  Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. 
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet