[Histonet] blurry tissue

Rene J Buesa rjbuesa <@t> yahoo.com
Mon Jul 25 15:45:42 CDT 2011


You just pointed out to the most likely causes: happening during staining, high humidity and water in the xylene. Try to take care of these issues and you will resolve the problem.
René J.

--- On Mon, 7/25/11, Carol Bryant <cbrya <@t> lexclin.com> wrote:


From: Carol Bryant <cbrya <@t> lexclin.com>
Subject: [Histonet] blurry tissue
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Monday, July 25, 2011, 1:42 PM


I would like some thoughts on how to resolve some "blurry" looking tissue.  We have had occasional tissue that looks blurry and not crisp for several weeks now.  It is not all the cases only random tissues.  The tissue is not on the same tissue processor either. We have 2 processors.  The latest cases were a breast, some skins, and a prostate.  I am not certain if this is happening on the tissue processor or in the stainer.  It has been very humid in our lab so I have started running a dehumidifier in case there is water in the xylene.  It is so hit and miss that I am puzzled.   Any suggestions would be greatly appreciated.
Thank you in advance for your thoughts.

Carol Bryant, CT (ASCP)
Cytology/Histology Manager
Pathology Services
Lexington Clinic
Phone (859) 258-4082
Fax (859) 258-4081
cbrya <@t> lexclin.com



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