[Histonet] blurry tissue

[DKBoyd <@t> chs.net]

Are you using recycled alcohols?   Check your concentrations.  Could your 
cassettes be "floating up" during processing?  It is possible they have 
air bubbles trapped in the cassette and aren't properly dehydrated.  Check 
and change your staining alcohols and clearants to make sure there isn't 
water from humidity or carry over.
It has always been my experience that recycled dehydrants/clearants are 
the culprit.  But, that being said, we have on occasions had an errant 
cassettes to float up during processing. 

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkboyd <@t> chs.net







Carol Bryant <cbrya <@t> lexclin.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
07/25/2011 01:42 PM

To
"histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
cc

Subject
[Histonet] blurry tissue






I would like some thoughts on how to resolve some "blurry" looking tissue. 
 We have had occasional tissue that looks blurry and not crisp for several 
weeks now.  It is not all the cases only random tissues.  The tissue is 
not on the same tissue processor either. We have 2 processors.  The latest 
cases were a breast, some skins, and a prostate.  I am not certain if this 
is happening on the tissue processor or in the stainer.  It has been very 
humid in our lab so I have started running a dehumidifier in case there is 
water in the xylene.  It is so hit and miss that I am puzzled.   Any 
suggestions would be greatly appreciated.
Thank you in advance for your thoughts.

Carol Bryant, CT (ASCP)
Cytology/Histology Manager
Pathology Services
Lexington Clinic
Phone (859) 258-4082
Fax (859) 258-4081
cbrya <@t> lexclin.com



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